Modulation of apoptotic pathways by curcumin and resveratrol conveys neuroprotection against rotenone-induced toxicity in SH-SY5Y cells

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dc.authorwosidMeltem Yılmaz Coşar / A-1458-2019
dc.contributor.authorGenç, Betül
dc.contributor.authorCoşar, Meltem Yılmaz
dc.contributor.authorErdoğan, Mümin Alper
dc.contributor.authorYılmaz, Özlem
dc.date.accessioned2025-04-18T06:56:36Z
dc.date.available2025-04-18T06:56:36Z
dc.date.issued2024
dc.departmentİstinye Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Anestezi Programı
dc.description.abstractAim: This study investigates the neuroprotective properties of the polyphenolic compounds resveratrol and curcumin against rotenone-induced toxicity in SH-SY5Y neuronal cells. Material and Methods: The cytotoxic threshold (LD50) of rotenone was established through a dose-response assessment in SH-SY5Y cells. Cells were pre- incubated with varying concentrations of resveratrol (1-100 mu M) and curcumin (10-1000 nM), followed by a 24-hour rotenone challenge (200 nM). Cell survival was gauged using the MTS assay, while caspase-3 levels, as a marker of apoptosis, were quantified via ELISA. The capability of cells to form colonies post-treatment was determined through a clonogenic survival assay. Moreover, the Western blot analysis revealed that pre-treatment with resveratrol and curcumin significantly modulated the protein expression levels of Bax and Bcl-2. Results: Pretreatment with resveratrol and curcumin significantly enhanced cell viability and clonogenic survival in the presence of rotenone, with notable decreases in caspase-3 levels, implying reduced apoptosis (p < 0.05). These compounds also modulated the expression of Bax and Bcl-2, contributing to their protective effects. Discussion: Resveratrol and curcumin exhibit promising neuroprotective effects by improving cell survival and modulating apoptotic markers in SH-SY5Y cells subjected to rotenone-induced toxicity. These findings support the potential therapeutic role of these polyphenols in neurodegenerative disease management, pending further validation through comprehensive pre-clinical and clinical studies.
dc.description.sponsorshipEge University
dc.identifier.citationGenc, B., Cosar, M. Y., Erdogan, M. A., & Yılmaz, O. Modulation of apoptotic pathways by curcumin and resveratrol conveys neuroprotection against rotenone-induced toxicity in SH-SY5Y cells.
dc.identifier.doi10.4328/ACAM.22183
dc.identifier.endpage574
dc.identifier.issn2667-663X
dc.identifier.issue8
dc.identifier.startpage570
dc.identifier.urihttp://dx.doi.org/10.4328/ACAM.22183
dc.identifier.urihttps://hdl.handle.net/20.500.12713/6397
dc.identifier.volume15
dc.identifier.wosWOS:001284430000011
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.institutionauthorCoşar, Meltem Yılmaz
dc.institutionauthoridMeltem Yılmaz Coşar / 0000-0001-5744-0937
dc.language.isoen
dc.publisherBayrakol medical publisher
dc.relation.ispartofAnnals of clinical and analytical medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCurcumin
dc.subjectResveratrol
dc.subjectRotenone
dc.subjectNeuroprotection
dc.subjectApoptosis
dc.subjectSH-SY5Y Cells
dc.titleModulation of apoptotic pathways by curcumin and resveratrol conveys neuroprotection against rotenone-induced toxicity in SH-SY5Y cells
dc.typeArticle

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