Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells

Basit Öğe Kaydı
dc.authoridÖter Gamze Nur / 0000-0002-7063-3984
dc.authoridEzgi Durmus / 0000-0002-0760-497X
dc.authoridAli Sen / 0000-0002-2144-5741
dc.authoridAbdurrahim Kocyigit / 0000-0003-2335-412X
dc.authorwosid
dc.contributor.authorNur, Öter Gamze
dc.contributor.authorDurmus, Ezgi
dc.contributor.authorSen, Ali
dc.contributor.authorKocyigit, Abdurrahim
dc.date.accessioned2025-04-18T09:18:29Z
dc.date.available2025-04-18T09:18:29Z
dc.date.issued01.11.2024
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü
dc.description.abstractObjective:Plants have been used for medicinal purposes since the beginning of human history and form the basis of modern medicine, and they are also the source of most chemotherapeutic drugs for cancer treatment. This study aims to investigate for the first time the cytotoxic and apoptotic effects of the active ethanol (RTE) and chloroform (RTC) extracts of Rubus tereticaulis leaves in the A549 non-small-cell lung cancer cell line. Materials and Methods:A549 cells were treated with RTE and RTC individually. The MTT assay was used to quantitatively detect RTE and RTC’s cytotoxic effects. The fluorescent signal indicator H2DCF-DA was used to detect cellular reactive oxygen species (ROS) production. Apoptosis was evaluated by fluorescence microscope after acridine orange/ethidium bromide fluorescent staining, annexin V-FITC and immunoblotting analyses, immunofluorescence, and imaging. Results:Both RTE and RTC induced cytotoxicity in A549 cells in a dose-dependently, which was accompanied with induced ROS accumulation. Both early and late apoptotic cells detected by flow cytometry were increased in the RTE- and RTC-treated cells. In addition, the results show RTC to have higher cytotoxic and apoptotic effects and increased ROS-generation capacity than RTE. Therefore, the polarity of the solvent used to exert the anticancer effect of R. tereticaulis leaves is crucial. Conclusion:This is the first anti-cancer activity study on R. tereticaulis. The results suggest R. tereticaulis leaves to have an anti-cancer effect on lung cancer cells through ROS-mediated apoptosis and RTC to be an effective therapeutic/adjuvant strategy in cancer treatment.
dc.identifier.citationNUR, Ö. G., NUR, Ö. G., DURMUS, E., DURMUS, E., SEN, A., KOCYIGIT, A. (2024). Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells. European Journal of Biology , 83(1), 50 - 59. doi.org/10.26650/EurJBiol.2024.1353842
dc.identifier.doi10.26650/EurJBiol.2024.1353842
dc.identifier.endpage59
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85195689649
dc.identifier.startpage50
dc.identifier.trdizinid1276776
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1276776/induction-of-apoptosis-through-oxidative-stress-caused-by-rubus-tereticaulis-leaves-extracts-in-a549-cells
dc.identifier.urihttps://hdl.handle.net/20.500.12713/6752
dc.identifier.volume83
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorNur, Öter Gamze
dc.institutionauthoridÖter Gamze Nur / 0000-0002-7063-3984
dc.language.isoen
dc.publisherİstanbul Üniversitesi Yayınevi
dc.relation.ispartofEuropean Journal of Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectLung cancer
dc.subjectanti-cancer effect
dc.subjectoxidative stress
dc.subjectRubus tereticaulis
dc.subjectapoptosis
dc.titleInduction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells
dc.typeArticle

Dosyalar

Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.17 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Cerrahi Tıp Bilimleri Bölümü Makale Koleksiyonu