Comparison of four phenotypic assays and check-direct CPE for detection of carbapenemases-producing enterobacterales

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dc.authoridAyham Abulaila / 0000-0001-9615-3391en_US
dc.authorscopusidAyham Abulaila / 57191905263
dc.authorwosidAyham Abulaila / AAV-4591-2020
dc.contributor.authorAbulaila, Ayham
dc.contributor.authorErdem, Fatma
dc.contributor.authorÖncül, Oral
dc.contributor.authorAktaş, Zerrin
dc.date.accessioned2021-08-01T07:21:27Z
dc.date.available2021-08-01T07:21:27Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractBackground: Early and accurate detection of carbapenemase-producing Enterobacterales (CPE) is fundamental to prevent their spread in hospital environment. Our objective was to compare between four commonly used phenotypic assays and Check-Direct CPE (CDCPE) multiplex PCR in CPE detection. We examined stool samples or rectal swabs for CPE, samples collected from 23 Jan 2017 to 23 Jul 2017 from patients in intensive-care units (ICUs) of our hospital. Methods: A panel of 98 non-repetitive Enterobacterales isolates with reduced susceptibility to carbapenems were analyzed by means of (i) Modified Hodge Test (MHT), (ii) Blue Carba test (BCT), (iii) Combined Disc Test (CDT), and (iv) The Carbapenem Inactivation Method (CIM). All these phenotypic tests compared with CDCPE. Confirmation and validation of results was achieved by classical PCR and sequencing. Results: Of the 98 non-repetitive Enterobacterales isolates, ninety-one were K. pneumoniae (93%), three K. oxytoca (3%), three E. cloacae (3%) and one E. coli (1%). By classic PCR the carbapenem resistance genes in K. pneumoniae isolates distributed as the followings; 49 blaOXA-48, 34 both blaOXA-48 and blaNDM-1, seven blaNDM-1 and one blaKPC. K. oxytoca; two blaOXA-48, one blaNDM-1. E. cloacae; two blaOXA-48, one blaNDM-1. E. coli; one isolate with both blaOXA-48 and blaNDM-1. The most common carbapenemase gene detected was blaOXA-48 rate of 54% (n = 53) followed by a combination of both blaOXA-48 and blaNDM-1 with rate of 36% (n = 35), only blaNDM-1 9% (n = 9) and blaKPC 1% (n = 1). Among phenotypic tests, we found CIM, MHT, and BCT correctly identified carbapenemase producers with sensitivity of 100%, 98%, and 90.8%, respectively. Conclusions: Rapid and accurate detection of CRE can be achieved by combination of both phenotypic and molecular tests. Surveillance studies are important both in terms of epidemiology and regulation of the treatment of patients. © 2021 Verlag Klinisches Labor GmbH. All rights reserved.en_US
dc.identifier.citationAbulaila, A., Erdem, F., Oncul, O., & Aktas, Z. (2021). Comparison of Four Phenotypic Assays and Check-Direct CPE for Detection of Carbapenemases-Producing Enterobacterales. Clinical Laboratory, 67(7).en_US
dc.identifier.doi10.7754/Clin.Lab.2020.201002en_US
dc.identifier.endpage1715en_US
dc.identifier.issn1433-6510en_US
dc.identifier.issue7en_US
dc.identifier.scopus2-s2.0-85109437647en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1707en_US
dc.identifier.urihttps://doi.org/10.7754/Clin.Lab.2020.201002
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1965
dc.identifier.volume67en_US
dc.identifier.wosWOS:000684711400024en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorAbulaila, Ayham
dc.language.isoenen_US
dc.publisherVerlag Klinisches Labor GmbHen_US
dc.relation.ispartofClinical Laboratoryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCarbapenemaseen_US
dc.subjectCheck-Direct CPEen_US
dc.subjectEnterobacteralesen_US
dc.subjectOXA-48/NDM/KPCen_US
dc.subjectPhenotypic Testsen_US
dc.subjectReal-Time PCRen_US
dc.titleComparison of four phenotypic assays and check-direct CPE for detection of carbapenemases-producing enterobacteralesen_US
dc.typeArticleen_US

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