Use of Liquid Culture with the ElecTIS Bioreactor for Faster Recovery of Blackberry (Rubus fruticosus L.) Shoots from Conservation at 4 °C

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dc.contributor.authorElazab, Doaa
dc.contributor.authorCapuana, Maurizio
dc.contributor.authorOzudogru, Elif Aylin
dc.contributor.authorAnichini, Monica
dc.contributor.authorLambardi, Maurizio
dc.date.accessioned2024-05-19T14:39:27Z
dc.date.available2024-05-19T14:39:27Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractElecTIS is a new single container bioreactor which does not require forced air blowing, instead making the culture material mobile and the liquid medium stationary. The timed up-and-down movement of the basket containing the shoot culture ensures periodic contact with the liquid medium positioned at the base of the container. In this study we tested for the first time its use in the recovery of blackberry shoot cultures (Rubus fruticosus L., cvs Thornfree and Chester), coming from 5 months of slow growth storage (SGS), at 4 & DEG;C and in the dark. The shoot recovery at standard culture conditions was performed on two different types of ElecTIS, i.e., one with a smaller basket (ElecTIS(S), 234 cm(2) of culture area), and one with a large basket (ElecTIS(L), 336 cm(2)), comparing the culture in TIS (cycle of 8 min every 6 h, equal to 32 min/day) with the traditional one in a gelled medium in glass jars (500 cc). After each one of the three 4-week subcultures, the shoot growth parameters and the relative growth rate highlighted a clear superiority of ElecTIS in promoting the recovery of shoot cultures coming from SGS. The analyses of chlorophyll content and stoma functionality confirmed the superior quality of shoots cultured in the ElecTIS bioreactor, and these shoots were afterwards easily rooted and acclimatized ex vitro.en_US
dc.description.sponsorshipInternational Treaty on Plant Genetic Resources for Food and Agriculture-RGV-FAO 7, 2023-2025 [50020, B97G23000120001]en_US
dc.description.sponsorshipThis research was funded by the International Treaty on Plant Genetic Resources for Food and Agriculture-RGV-FAO 7, 2023-2025. Ministerial Decree n 50020 of 01/02/2023, CUP n B97G23000120001.en_US
dc.identifier.doi10.3390/horticulturae9060680
dc.identifier.issn2311-7524
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85163743892en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org10.3390/horticulturae9060680
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4782
dc.identifier.volume9en_US
dc.identifier.wosWOS:001017149700001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofHorticulturaeen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectBioreactoren_US
dc.subjectBlackberryen_US
dc.subjectElectisen_US
dc.subjectLiquid Cultureen_US
dc.subjectRubus Fruticosusen_US
dc.subjectSlow Growth Storageen_US
dc.subjectTemporary Immersion Systemen_US
dc.titleUse of Liquid Culture with the ElecTIS Bioreactor for Faster Recovery of Blackberry (Rubus fruticosus L.) Shoots from Conservation at 4 °Cen_US
dc.typeArticleen_US

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