Multilayered nanocomposite membrane orchestrating targeted dual release strategies for enhanced guided bone regeneration

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dc.authoridZarrabi, Ali/0000-0003-0391-1769
dc.authorwosidZarrabi, Ali/U-2602-2019
dc.contributor.authorAssadi, Zahra
dc.contributor.authorRezvanian, Parsa
dc.contributor.authorGounani, Zahra
dc.contributor.authorEjeian, Fatemeh
dc.contributor.authorZarrabi, Ali
dc.contributor.authorMasaeli, Elahe
dc.date.accessioned2024-05-19T14:42:37Z
dc.date.available2024-05-19T14:42:37Z
dc.date.issued2024
dc.departmentİstinye Üniversitesien_US
dc.description.abstractPresently, guided bone regeneration (GBR) stands as a preeminent strategy for rectifying periodontal and maxillofacial bone imperfections. Nonetheless, the current GBR membranes encounter limitations in terms of antibacterial efficacy and osteogenic potential, impeding their overall effectiveness. To address this, our study endeavors to engineer an innovative GBR membrane with dual attributes of osteoinductiveness and antibacterial activity. This novel asymmetric nanocomposite integrates an intricate structure comprising a compact chitosan hydrogel layer embedded with tetracycline (TCH) and a porous polycaprolactone fibrous layer incorporating chitosan nanoparticles loaded with icariin (IcA@CNps). In the initial phase, IcA@CNps, exhibiting a balanced CNps to IcA ratio of 1:2, an average dimension of 80.94 +/- 33.78 nm, and a zeta potential of -19.3 mV, were synthesized and affixed onto nanofibers. This integration facilitated the controlled release of icariin over a span of 22 days. Subsequently, the asymmetric nanocomposite GBR membrane (referred to as GBR+) was meticulously crafted. This involved the application of a TCH hydrogel laden with a drug concentration of 120 mu g/ml onto the IcA@CNps-coated nanofibrous layer through an intermediary hydrogel stratum. Impressive results were witnessed during in vitro trials against Staphylococcus aureus, showcasing the remarkable antibacterial prowess of the GBR+ membrane. Notably, human alveolar bone marrow stem cells (aBMSCs) cultivated on the GBR+ membrane exhibited favorable cell adhesion and proliferation rates, devoid of any cytotoxic manifestations. Gene expression analyses further unveiled elevated expressions of SPP1, BMP2, and RUNX2 in the proximity of the fabricated membrane, indicating the promotion of bone formation pathways. Consequently, this asymmetric GBR membrane presents a promising therapeutic avenue for bolstering bone regeneration, underscored by its dual action of fostering bone growth while concurrently displaying antibacterial attributes.en_US
dc.identifier.doi10.1016/j.cej.2024.149237
dc.identifier.issn1385-8947
dc.identifier.issn1873-3212
dc.identifier.scopus2-s2.0-85185594717en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org10.1016/j.cej.2024.149237
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5263
dc.identifier.volume484en_US
dc.identifier.wosWOS:001197154700001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Saen_US
dc.relation.ispartofChemical Engineering Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectIcariinen_US
dc.subjectOsteogenesisen_US
dc.subjectChitosan Nanoparticleen_US
dc.subjectNanofibersen_US
dc.subjectGuided Bone Regeneration (Gbr)en_US
dc.titleMultilayered nanocomposite membrane orchestrating targeted dual release strategies for enhanced guided bone regenerationen_US
dc.typeArticleen_US

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