The effect of lidocaine on TRPM 2,6,7 and 8 channels in liver ischemia / reperfusion model in rats
| dc.authorid | Tuna Bilecik / 0000-0002-7160-2127 | en_US |
| dc.authorid | Ömer Vefik Özozan / 0000-0001-5885-7865 | en_US |
| dc.authorscopusid | Tuna Bilecik / 55758337600 | |
| dc.authorscopusid | Ömer Vefik Özozan / 20434255000 | |
| dc.authorwosid | Tuna Bilecik / ELH-9331-2022 | |
| dc.authorwosid | Ömer Vefik Özozan / AAU-4795-2020 | |
| dc.contributor.author | Bilecik, Tuna | |
| dc.contributor.author | Özozan, Ömer Vefik | |
| dc.contributor.author | Gökçe, Hasan | |
| dc.date.accessioned | 2020-10-08T11:24:27Z | |
| dc.date.available | 2020-10-08T11:24:27Z | |
| dc.date.issued | 2020 | en_US |
| dc.department | İstinye Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü | en_US |
| dc.description.abstract | Aim: The transient receptor potential melastatin (TRPM) channel is subfamilies of the transient receptor potential (TRP) channels, cation permeable channels. Ischemia and reperfusion (I / R) injury is a phenomenon highlighting cellular damage of the liver caused by oxygen. Lidocaine is a local anesthetic that blocks sodium channels and suppresses mitochondrial functions of neutrophils. In this study, we purpose to investigate the effects of TRPM2/8 and TRPM6/7 expressions after lidocaine treatment in liver ischemia/reperfusion rat model. Materials and Methods: The study was carried out on 32 male Wistar rats. The animals were randomly divided into 4 groups including sham, lidocaine, I/R group and I/R- lidocaine group. The portal vein and the hepatic artery branches were clamped for 60 minutes for complete ischemia. TRPM2/8 and TRPM6/7 gene expression levels were assessed by RT-qPCR. Results: The expression levels of TRPM2 were significantly higher in liver-I/R group compared to sham and lidocaine groups (p<0.05, p<0.000, respectively), TRPM8 genes were significantly higher in liver-I/R group compared to sham and lidocaine groups (p<0.05, p<0.05, respectively). However, the expression levels of TRPM6 gene was significantly higher in liver-I/R group compared to sham, lidocaine, and I/R-lidocaine groups (p<0.05, p<0.01, p<0.05, respectively), TRPM7 genes was significantly higher in liver-I/R group compared to sham, lidocaine and I/R-lidocaine groups (p<0.05, p<0.000, p<0.05, respectively). Discussion: In conclusion, we firstly showed that an association between the expression level of TRPM2/8, TRPM6/7, and hepatic I/R, I/R-lidocaine groups as well as TRPM2/8 and TRPM6/7 gene expressions are affected by lidocaine in the liver-I/R in a rat model. | en_US |
| dc.identifier.citation | Bilecik, T., Ozozan, O. V., & Gokce, H. The effect of lidocaine on TRPM 2, 6, 7 and 8 channels in liver ischemia/reperfusion model in rats. | en_US |
| dc.identifier.doi | 10.4328/ACAM.20075 | en_US |
| dc.identifier.endpage | 324 | en_US |
| dc.identifier.issn | 2667-663X | en_US |
| dc.identifier.issue | 4 | en_US |
| dc.identifier.startpage | 319 | en_US |
| dc.identifier.uri | https://doi.org/10.4328/ACAM.20075 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12713/1134 | |
| dc.identifier.volume | 11 | en_US |
| dc.identifier.wos | WOS:000572683500016 | en_US |
| dc.identifier.wosquality | N/A | en_US |
| dc.indekslendigikaynak | Web of Science | en_US |
| dc.institutionauthor | Bilecik, Tuna | |
| dc.institutionauthor | Özozan, Ömer Vefik | |
| dc.language.iso | en | en_US |
| dc.publisher | BAYRAKOL MEDICAL PUBLISHER | en_US |
| dc.relation.ispartof | ANNALS OF CLINICAL AND ANALYTICAL MEDICINE | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Sodium Channel | en_US |
| dc.subject | Ischemia | en_US |
| dc.subject | Reperfusion | en_US |
| dc.subject | TRPM | en_US |
| dc.subject | Lidocaine | en_US |
| dc.title | The effect of lidocaine on TRPM 2,6,7 and 8 channels in liver ischemia / reperfusion model in rats | en_US |
| dc.type | Article | en_US |
