Establishment of an Efficient Somatic Embryogenesis Protocol for Giant Reed (Arundo donax L.) and Multiplication of Obtained Shoots via Semi-Solid or Liquid Culture

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dc.authoridKARLIK, Elif/0000-0003-0669-2725
dc.contributor.authorOzudogru, Elif Aylin
dc.contributor.authorKarlik, Elif
dc.contributor.authorElazab, Doaa
dc.contributor.authorLambardi, Maurizio
dc.date.accessioned2024-05-19T14:41:13Z
dc.date.available2024-05-19T14:41:13Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractThis study developed an efficient protocol for the in vitro propagation of giant reed (Arundo donax L.) biomass, defining a complete cycle of the induction of somatic embryogenesis from immature inflorescences, followed by the maturation of somatic embryos and the subsequent multiplication of the derived shoots in liquid culture in a temporary immersion system (TIS). The best explants were found to be 30 cm long immature inflorescences, preferably collected in spring. Such an explant type was easy to decontaminate, and the spikelets isolated from it provided over 100 embryogenic callus lines. Among the callus induction media tested, gelled MS medium supplemented with 1.1 mg/L 2,4-D provided the highest percentage of responsive spikelets and the highest density of embryogenic callus. Maturation of the embryogenic callus was easily triggered on gelled MS medium devoid of plant growth regulators. The obtained shoots could be further multiplied on previously optimized gelled DKW medium supplemented with 30 g/L sucrose, 5 mg/L BA, 0.1 mg/L IBA, and 6.8 g/L plant agar. Subsequent high multiplication of the developed shoots was achieved in liquid culture in TIS using a Plantform & TRADE; bioreactor, with an immersion cycle of 12 min every 8 h.en_US
dc.description.sponsorshipInternational Treaty on Plant Genetic Resources for Food and Agriculture-RGV-FAO 6, 2020-2022; Istinye University (Istanbul, Turkey)en_US
dc.description.sponsorshipThis research was funded by the International Treaty on Plant Genetic Resources for Food and Agriculture-RGV-FAO 6, 2020-2022. This work was also supported by Istinye University (Istanbul, Turkey).en_US
dc.identifier.doi10.3390/horticulturae9070735
dc.identifier.issn2311-7524
dc.identifier.issue7en_US
dc.identifier.scopus2-s2.0-85166321612en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org10.3390/horticulturae9070735
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5079
dc.identifier.volume9en_US
dc.identifier.wosWOS:001038519500001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofHorticulturaeen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectEmbryogenic Callusen_US
dc.subjectGiant Reeden_US
dc.subjectImmature Inflorescencesen_US
dc.subjectIndirect Somatic Embryogenesisen_US
dc.subjectPerennial Grassen_US
dc.titleEstablishment of an Efficient Somatic Embryogenesis Protocol for Giant Reed (Arundo donax L.) and Multiplication of Obtained Shoots via Semi-Solid or Liquid Cultureen_US
dc.typeArticleen_US

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