Functionalization of metal-organic frameworks with metallic nanoclusters for ultra-sensitive monitoring of morphine in biological fluids
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Sa
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A reliable, fast, and cost-effective method of monitoring morphine (MO) concentration in biological fluids is highly desired in clinical and modern medicine. Nanotechnology has opened up new possibilities for developing morphine biosensors, but the most advanced electrodes available offer a detection limit (LOD) of >= 10 nM. This work introduces a new, ultrasensitive electrochemical biosensor based on microporous metal-organic frameworks (-1100 cm2 g-1 surface area) modified by Ag clusters (-2 nm) and functionalized by Au nanoparticles (-180 nm). Electrochemical studies show that functionalizing the microporous crystalline particles (620 +/- 45 nm) with metal nanoclusters enhances charge transfer kinetics and electrochemical surface area by about 50 and 10 folds, respectively, resulting in high sensitivity (0.127 mu A mu M-1), broad linear dynamic range (0.05-600 mu M), and low LOD (3 +/- 0.2 nM). The differential pulse voltammetry confirms the stability (over 6 weeks), repeatability (RSD=3.0%), and reproducibility (RSD=4.0%) of the hybrid biosensor. The decent selectivity and specificity (RSD=7.4%) of the biosensor against various analogs co-existing with MO in urine have also been determined. Therefore, the hybrid metal-inorganic biosensor presents a promising prospect for simple, lowcost, and precise monitoring of biomolecules, including morphine, in biological fluids and clinical medicine.
Açıklama
Anahtar Kelimeler
Microporous Electroactive Materials, Hybrid Metal-Organic Composites, Bimetallic Noble Metal Clusters, Electrochemical Biosensor
Kaynak
Sensors and Actuators B-Chemical
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
393
