Thymoquinone reduces methotrexate-induced heart damage: a histopathological study in rats

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dc.authoridCEYLAN, TAYFUN/0000-0002-0917-0378
dc.authoridKarabulut, Derya/0000-0002-1903-9525
dc.authoridÖNAL, Esra/0000-0002-2631-6033
dc.authorwosidCEYLAN, TAYFUN/AAF-1001-2021
dc.authorwosidKarabulut, Derya/D-8233-2019
dc.authorwosidÖNAL, Esra/JQV-4238-2023
dc.contributor.authorYildirim, Aysegul Burcin
dc.contributor.authorKaymak, Emin
dc.contributor.authorCeylan, Tayfun
dc.contributor.authorAkin, Ali Tugrul
dc.contributor.authorKuloglu, Nurhan
dc.contributor.authorSayan, Meryem
dc.contributor.authorDeger, Necla
dc.date.accessioned2024-05-19T14:40:24Z
dc.date.available2024-05-19T14:40:24Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractPurpose: The study aimed to evaluate the effect of thymoquinone on cardiac tissue in MTX-induced cardiac toxicity in rats with various parameters.Materials and Methods: Group I (n=8) was administered intraperitoneal saline for 10 days. Intraperitoneal olive oil was applied to Group II (n=8) for 10 days. Group III (n=8) received 10 mg/kg Thymoquinone (THQ) intraperitoneally for 10 days. Group IV (n=8) was administered a single dose of 20 mg/kg Methotrexate (MTX), 500 mg/20 ml, intraperitoneally on the 1st day of the experiment. Group V (n=8) MTX: 20 mg/kg single dose intraperitoneally on the 1st day; THQ: 10mg/kg i.p. administered for 10 days. Since Methotrexate was in liquid form, no solvent was used. At the end of the experimental period, the rats were sacrificed for analysis of heart tissue. The structure of heart tissue was evaluated by hematoxylin-eosin staining. Immunohistochemically, Connexin-43, HSP90, and HIF- 1 alpha antibodies were stained.Results: Group IV was found to have histopathological deterioration, which was ameliorated by THQ. In addition to this; Connexin-43 immunoreactivity was the lowest in Group IV compared to other groups: 108.5 +/- 7.4. Compared to other groups, HSP90 immunoreactivity was highest in Group IV: 103.6 +/- 10.4. Compared to other groups, HIF-1 alpha immunoreactivity was highest in Group IV: 95.2 +/- 9.1.Conclusion: Thymoquinone has a positive effect on Connexin-43, one of the proteins providing conduction in intercalary discs, HSP90, one of the chaperones in the cell and HIF-1 alpha expression against MTX toxicity. At the same time, THQ provides a significant improvement in cardiac tissue histopathologically by showing a cardioprotective effect.en_US
dc.description.sponsorshipYozgat Bozok University Scientific Research Projects (BAP) [21/036]en_US
dc.description.sponsorshipThis study was supported by Yozgat Bozok University Scientific Research Projects (BAP) Coordinatorship with project number 21/036.en_US
dc.identifier.doi10.17826/cumj.1314101
dc.identifier.endpage851en_US
dc.identifier.issn2602-3032
dc.identifier.issn2602-3040
dc.identifier.issue3en_US
dc.identifier.startpage844en_US
dc.identifier.trdizinid1199190en_US
dc.identifier.urihttps://doi.org10.17826/cumj.1314101
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1199190
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4954
dc.identifier.volume48en_US
dc.identifier.wosWOS:001117679900017en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherCukurova Univ, Fac Medicineen_US
dc.relation.ispartofCukurova Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectConnexin-43en_US
dc.subjectHsp90en_US
dc.subjectHif-1 Alphaen_US
dc.subjectMethotrexateen_US
dc.subjectThymoquinoneen_US
dc.titleThymoquinone reduces methotrexate-induced heart damage: a histopathological study in ratsen_US
dc.typeArticleen_US

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