Cotton biotechnology: An efficient gene transfer protocol via agrobacterium tumefaciens for a greater transgenic recovery

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dc.authoridNermin Gözükırmızı / 0000-0002-7129-3045
dc.authorscopusidNermin Gözükırmızı / 6701520637
dc.authorwosidNermin Gözükırmızı / M-1419-2013
dc.date.accessioned2022-02-28T14:13:31Z
dc.date.available2022-02-28T14:13:31Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.description.abstractDue to its economic worth, cotton (Gossypium hirsutum L.) is grown in almost 70 countries and provides income for more than 250 million people. Therefore, producing cotton with having some desired characteristics that includes extended biotic and abiotic stress tolerance, improved fiber quality, promoted nutritional content and increased yield is the main objective for cotton biotechnology. To achieve this goal, many tissue culture and gene transfer techniques are being developed and used throughout the years. As applications for the gene transfer, the Agrobacterium-mediated, particle bombardment and pollen tube pathway-mediated methods are most successfully used and in conjunction with this, meristematic shoot tips as explants are efficiently utilized in gene transfer methods. In this study, the main objective was to report an efficient protocol for a greater recovery of transgenic cotton plant using Agrobacterium tumefaciens-mediated transformation. For this, one of the cotton strains (Cukurova 1518) cultivated widely in Turkey was chosen and meristematic shoot tips as explant sources, and GFP and NPTII genes as reporter and marker genes were used, respectively. The effective post co-cultivation conditions were provided via using the selection regime in vitro. Finally, the current results showed highly reproducible protocol developed could be used to produce transgenic cotton plants expressing desired traits or can be utilized as a model system to study the expression of particular genes.en_US
dc.identifier.citationOzyigit, I. I., Dogan, I., Kaya, Y., Bajrovic, K., & Gozukirmizi, N. (2022). Cotton Biotechnology: An Efficient Gene Transfer Protocol via Agrobacterium tumefaciens for a Greater Transgenic Recovery. Journal of Natural Fibers, 1-15.en_US
dc.identifier.doi10.1080/15440478.2022.2029662en_US
dc.identifier.issn1544-0478en_US
dc.identifier.issn1544-046Xen_US
dc.identifier.scopus2-s2.0-85125287576en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1080/15440478.2022.2029662
dc.identifier.urihttps://hdl.handle.net/20.500.12713/2511
dc.identifier.wosWOS:000754170400001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorGözükırmızı, Nermin
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS INCen_US
dc.relation.ispartofJOURNAL OF NATURAL FIBERSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGossypium Hirsutum Len_US
dc.subjectGene Transferen_US
dc.subjectMeristematic Shooten_US
dc.subjectBlotting Techniquesen_US
dc.titleCotton biotechnology: An efficient gene transfer protocol via agrobacterium tumefaciens for a greater transgenic recoveryen_US
dc.typeReview Articleen_US

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