Generation of an in vitro intratumoral heterogeneity model by lentiviral fluorescent labeling of colon cancer cell line dld-1 subclones

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dc.authoridÖykü Gönül Geyik / 0000-0003-3014-1253
dc.authorscopusidÖykü Gönül Geyik / 56695248800
dc.authorwosidÖykü Gönül Geyik / AHB-4716-2022
dc.contributor.authorGönül Geyik, Öykü
dc.contributor.authorEfe, Hande
dc.contributor.authorKöse, Seda Baykal
dc.contributor.authorUysal, Özge
dc.contributor.authorYüce, Zeynep
dc.date.accessioned2020-08-30T20:06:19Z
dc.date.available2020-08-30T20:06:19Z
dc.date.issued2020
dc.departmentİstinye Üniversitesi, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümüen_US
dc.descriptionGeyik, Oyku Gonul/0000-0003-3014-1253; Baykal Kose, Seda/0000-0001-8654-5332en_US
dc.description.abstractPurpose: Studying genomic changes during tumor progression has helped to understand the biology of many different cancers and has been the basis for targeted therapy strategies. However, resistance and differences in response to therapy in patients are still very important issues. One of the major underlying reasons is intratumoral cellular heterogeneity. Clones harbor mutations and/or epigenetic patterns providing a survival advantage under changing micro-environmental conditions are the main culprits of therapy resistance. Therefore, it is crucial to define and to study the properties and the contributions of these deviant subclones in vitro. In order to achieve that, we have generated a fluorescent intratumoral heterogeneity model of the colon cancer cell line DLD-1. Methods: We used 2N subclones (C3 and C34) and 4N subclones (B9 and B12) of DLD-1, isolated by our team previously. Subclones were stably transduced using lentiviral vectors carrying different fluorescent labels and selected by puromycin. Results: Labeled subclones were mixed in equal proportions and a co-culture model of intratumoral heterogeneity was generated. Fluorescent signals were then confirmed by fluorescence microscope. Conclusion: The in vitro model we have generated may be used in many tumor kinetic studies. By co-culturing different clones, profiles that have a selective advantage under different conditions can be detected. After exposure to different chemotherapeutic agents, radiation and/or combinations, real time changes in population kinetics can be tracked. By comparing these results to the genomic profiling of subclones, it will be possible to relate variations that are responsible for any observed therapeutic resistance in vitro.en_US
dc.description.sponsorshipTUBITAK 1001 Research GrantTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [116Z300]en_US
dc.description.sponsorshipThis study was funded by TUBITAK 1001 Research Grant; 116Z300.en_US
dc.identifier.citationGeyik, O. G., Efe, H., Kose, S. B., Uysal, O., & Yuce, Z. (2020). Generation of an in vitro Intratumoral Heterogeneity Model by Lentiviral Fluorescent Labeling of Colon Cancer Cell Line DLD-1 Subclones. JOURNAL OF BASIC AND CLINICAL HEALTH SCIENCES, 4(2), 149–153. https://doi.org/10.30621/jbachs.2020.921en_US
dc.identifier.doi10.30621/jbachs.2020.921en_US
dc.identifier.endpage153en_US
dc.identifier.issn2458-8938en_US
dc.identifier.issn2564-7288en_US
dc.identifier.issue2en_US
dc.identifier.startpage149en_US
dc.identifier.urihttps://doi.org/10.30621/jbachs.2020.921
dc.identifier.urihttps://hdl.handle.net/20.500.12713/470
dc.identifier.volume4en_US
dc.identifier.wosWOS:000533600300012en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.institutionauthorGönül Geyik, Öyküen_US
dc.language.isoenen_US
dc.publisherDokuz Eylul Univ Inst Health Sciencesen_US
dc.relation.ispartofJournal of Basic and Clinical Health Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectColon Canceren_US
dc.subjectDld-1en_US
dc.subjectFluorescent Labelingen_US
dc.subjectLentiviral Transductionen_US
dc.subjectCancer Therapy Resistanceen_US
dc.titleGeneration of an in vitro intratumoral heterogeneity model by lentiviral fluorescent labeling of colon cancer cell line dld-1 subclonesen_US
dc.typeArticleen_US

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