Standardization of gut microbiota analysis: variability in samples taken at different times from single case and the effect of the freezing the sample

Basit Öğe Kaydı
dc.authoridFiliz Yarımcan Sağlam / 0000-0001-8267-0435
dc.authorscopusidFiliz Yarımcan Sağlam / 57199153940
dc.authorwosidFiliz Yarımcan Sağlam / IWD-5094-2023
dc.contributor.authorSağlam Yarımcan, Filiz
dc.date.accessioned2020-08-30T20:07:25Z
dc.date.available2020-08-30T20:07:25Z
dc.date.issued2018
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractBackground: Gut microbiota analysis can help understand many diseases some of which, like in?ammatory bowel diseases, obesityanddiabetesarealreadyestablishedandtheothersaretobe determined.Usingthistestfordiagnosticpurposesandestablishing personalizedcareseemstobecomearoutineprocedurethatneeds standardizationprotocols.Theaimofthisstudywastoanalyzethe changesingutmicrobiotacommunitiesinsamplestakenfromsingle case at different times and the effect of storing the samples in -20?C before processing them. Methods&Materials:Ontheday1andtheday8,twostoolsamples were obtained from a single case, 40-year-old woman using insulin for the control of her Type 1 Diabetes Mellitus. The ?rst sample were processed in 2hours and part of it was kept in -20 until day 8. The second stool sample was processed together with the frozen sample in 2hours. DNA was obtained with fecal DNA extraction kit (QIAGENE). The microbiota was determined using Ion16SrRNAMetagenomicKitonIonTorrentplatform.Theextractionweredoneasmentionedaboveandmetagenomicstudieswere doneforallsamplesinasinglerunandchip.Thecomparisonofthe results was done using t-test with SPSS (IBM) software. Results: Six phyla were determined: Bacteriodetes, Firmicutes, Proteobacteria, Tenericutes, Actinobacteria and Verrucomicrobia. Bacteriodetes and Firmicutes formed the majority of the bacterial population studied. Bacteriodetes were 57% in the 1st day fresh sample and 28% in the frozen one. Bacteriodetes ratio in 8th day sample was 44%. Firmicutes formed 35% in 1st day fresh sample, 38%and19%infrozenand8thdaysamplesrespectively.Proteobacteria ratios were high in frozen and 8th day samples: 31% and 36%. Proteobacteria formed 7% of bacterial population in the 1st dayfreshsample.Tenericutes,ActinobacteriaandVerrucomicrobia were below 1% in all samples.en_US
dc.identifier.citationSaglam, F. (2018). Standardization of gut microbiota analysis: Variability in samples taken at different times from single case and the effect of the freezing the sample. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 73(S), 170. https://doi.org/10.1016/j.ijid.2018.04.3799en_US
dc.identifier.doi10.1016/j.ijid.2018.04.3799en_US
dc.identifier.endpage170en_US
dc.identifier.issn1201-9712en_US
dc.identifier.issn1878-3511en_US
dc.identifier.startpage170en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijid.2018.04.3799
dc.identifier.urihttps://hdl.handle.net/20.500.12713/762
dc.identifier.volume73en_US
dc.identifier.wosWOS:000440345100365en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.institutionauthorSağlam Yarımcan, Filizen_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofInternational Journal of Infectious Diseasesen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleStandardization of gut microbiota analysis: variability in samples taken at different times from single case and the effect of the freezing the sampleen_US
dc.typeConference Objecten_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
61a.pdf
Boyut:
168.5 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Temel Tıp Bilimleri Bölümü Bildiri Koleksiyonu