Can Wharton jelly derived or adipose tissue derived mesenchymal stem cell can be a treatment option for duchenne muscular dystrophy? answers as transcriptomic aspect

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dc.authoridEda Sun / 0000-003-0320-5784en_US
dc.authoridErdal Karaöz / 0000-0002-9992-833Xen_US
dc.authorscopusidErdal Karaöz / 7003448087
dc.authorscopusidEda Sun / 57225054414
dc.authorwosidErdal Karaöz / IXE-6677-2023
dc.authorwosidEda Sun / GER-0120-2022
dc.contributor.authorSun, Eda
dc.contributor.authorKaraöz, Erdal
dc.date.accessioned2020-09-18T09:06:14Z
dc.date.available2020-09-18T09:06:14Z
dc.date.issued2020en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractIntroduction: Mesenchymal stem cells (MSCs) are able to differentiate into several cell lineages including skeletal muscle. In addition to their differentiation capacities, they have the ability to transfer their content genomic information horizontally through their exosomes and fusion abilities, as we have shown in our previous clinic study on Duchenne Muscular Dystrophy (DMD) patients, dystrophin expression increased after MSC treatment. Therefore, this study aimed to compare the transcriptomic properties of Wharton's jelly derived (WJ-) MSC and Adipose tissue (AT-) derived MSC, which are the two most preferred sources in MSC treatments applied in DMD. Methods: Both MSC cell lines obtained from ATCC (PCS-500-010; PCS-500-011) were characterized by flow cytometry then WJ-MSC and AT-MSC cell lines were sequenced via RNA-SEQ. R language was used to obtain the differentially expressed genes (DEGs) and differentially expressed miRNAs, respectively. Additionally, in order to support the results of our study, a gene expression profile data set of DMD patients (GSE1004) were acquired from Gene Expression Omnibus (GEO) database. Results: Here, we demonstrated that activated WNT signaling and downregulated TGF-? pathways under the control of decreased mir-24 which are involved in myogenic differentiation are differentially expressed in WJ-MSC. We have shown that the expression of mir-199a-5p, which is known to increase in exosomes of DMD patients, is less in WJ-MSC. Additionally, we have shown activated PI3K/Akt pathway, which is controlling mitochondria transfer via Tunnelling Nanotube as a new perspective in cellular therapies in myodegenerative diseases, in WJ-MSC more than in AT-MSCs. Conclusion: Summing up, WJ-MSC, which we recommend as an appropriate source candidate due to its immune-regulation properties, stands forward as a preferable source in the cellular treatment of DMD patients due to its transcriptomic aspect.en_US
dc.identifier.citationSun, E., & Karaoz, E. (2020). Can Wharton jelly derived or adipose tissue derived mesenchymal stem cell can be a treatment option for duchenne muscular dystrophy? Answers as transcriptomic aspect. American journal of stem cells, 9(4), 57–67.en_US
dc.identifier.endpage67en_US
dc.identifier.issn2160-4150en_US
dc.identifier.issue4en_US
dc.identifier.pmid32929392en_US
dc.identifier.startpage57en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1026
dc.identifier.volume9en_US
dc.identifier.wosWOS:000577119400001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorSun, Eda
dc.institutionauthorKaraöz, Erdal
dc.language.isoenen_US
dc.relation.ispartofAm J Stem Cellsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDuchenne Muscular Dystrophyen_US
dc.subjectGene Expressionen_US
dc.subjectMesenchymal Etem Cellsen_US
dc.subjectCellular Therapyen_US
dc.titleCan Wharton jelly derived or adipose tissue derived mesenchymal stem cell can be a treatment option for duchenne muscular dystrophy? answers as transcriptomic aspecten_US
dc.typeArticleen_US

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