Reduction of inflammation and enhancement of motility after pancreatic islet derived stem cell transplantation following spinal cord injury

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dc.authoridErdal Karaöz / 0000-0002-9992-833X
dc.authorscopusidErdal Karaöz / 7003448087
dc.authorwosidErdal Karaöz / IXE-6677-2023
dc.contributor.authorKaraöz, Erdal
dc.contributor.authorTepeköy, Filiz
dc.contributor.authorYılmaz, İrem
dc.contributor.authorSubaşı, Cansu
dc.contributor.authorKabataş, Serdar
dc.date.accessioned2020-08-30T20:06:55Z
dc.date.available2020-08-30T20:06:55Z
dc.date.issued2019
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.descriptionWOS: 000466716300004en_US
dc.description.abstractObjective : Spinal cord injury (SCI) is a very serious health problem, usually caused by a trauma and accompanied by elevated levels of inflammation indicators. Stem cell-based therapy is promising some valuable strategies for its functional recovery. Nestin-positive progenitor and/or stem cells (SC) isolated from pancreatic islets (PI) show mesenchymal stem cell (MSC) characteristics. For this reason, we aimed to analyze the effects of rat pancreatic islet derived stem cell (rPI-SC) delivery on functional recovery, as well as the levels of inflammation factors following SCI. Methods : rPI-SCs were isolated, cultured and their MSC characteristics were determined through flow cytometry and immunofluorescence analysis. The experimental rat population was divided into three groups : 1) laminectomy & trauma, 2) laminectomy & trauma & phosphate-buffered saline (PBS), and 3) laminectomy+trauma+SCs. Green fluorescent protein (GFP) labelled rPI-SCs were transplanted into the injured rat spinal cord. Their motilities were evaluated with Basso, Beattie and Bresnahan (BBB) Score. After 4-weeks, spinal cord sections were analyzed for GFP labeled SCs and stained for vimentin, S100 beta, brain derived neurotrophic factor (BDNF), 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNPase), vascular endothelial growth factor (VEGF) and proinflammatory (interleukin [IL]-6, transforming growth factor [TGF]-beta, macrophage inflammatory protein [MIP]-2, myeloperoxidase [MPO]) and anti-inflammatory (IL-1 receptor antagonis) factors. Results : rPI-SCs were revealed to display MSC characteristics and express neural and glial cell markers including BDNF, glial fibrillary acidic protein (GFAP), fibronectin, microtubule associated protein-2a,b (MAP2a,b), beta 3-tubulin and nestin as well as anti-inflammatory prostaglandin E2 receptor, EP3. The BBB scores showed significant motor recovery in group 3. GFP-labelled cells were localized on the injury site. In addition, decreased proinflammatory factor levels and increased intensity of anti-inflammatory factors were determined. Conclusion : Transplantation of PI-SCs might be an effective strategy to improve functional recovery following spinal cord trauma.en_US
dc.identifier.citationKaraoz, E., Tepekoy, F., Yilmaz, I., Subasi, C., & Kabatas, S. (2019). Reduction of Inflammation and Enhancement of Motility after Pancreatic Islet Derived Stem Cell Transplantation Following Spinal Cord Injury. JOURNAL OF KOREAN NEUROSURGICAL SOCIETY, 62(2), 153–165. https://doi.org/10.3340/jkns.2018.0035en_US
dc.identifier.doi10.3340/jkns.2018.0035en_US
dc.identifier.endpage165en_US
dc.identifier.issn2005-3711en_US
dc.identifier.issn1598-7876en_US
dc.identifier.issue2en_US
dc.identifier.pmid30840970en_US
dc.identifier.startpage153en_US
dc.identifier.urihttps://doi.org/10.3340/jkns.2018.0035
dc.identifier.urihttps://hdl.handle.net/20.500.12713/654
dc.identifier.volume62en_US
dc.identifier.wosWOS:000466716300004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaraöz, Erdalen_US
dc.language.isoenen_US
dc.publisherKorean Neurosurgical Socen_US
dc.relation.ispartofJournal of Korean Neurosurgical Societyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSpinal Corden_US
dc.subjectWounds And Injuriesen_US
dc.subjectIslets Of Langerhansen_US
dc.subjectStem Cellsen_US
dc.subjectRegenerationen_US
dc.titleReduction of inflammation and enhancement of motility after pancreatic islet derived stem cell transplantation following spinal cord injuryen_US
dc.typeArticleen_US

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