Evaluation of Antiproliferative Properties of CoMnZn-Fe2O4 Ferrite Nanoparticles in Colorectal Cancer Cells

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dc.contributor.authorNarayanaswamy, Venkatesha
dc.contributor.authorRah, Bilal
dc.contributor.authorAl-Omari, Imaddin A.
dc.contributor.authorKamzin, Alexander S.
dc.contributor.authorKhurshid, Hafsa
dc.contributor.authorMuhammad, Jibran Sualeh
dc.contributor.authorObaidat, Ihab M.
dc.date.accessioned2024-05-19T14:42:36Z
dc.date.available2024-05-19T14:42:36Z
dc.date.issued2024
dc.departmentİstinye Üniversitesien_US
dc.description.abstractThe PEG-coated ferrite nanoparticles Co0.2Mn0.6Zn0.2Fe2O4 (X1), Co0.4Mn0.4Zn0.2Fe2O4 (X2), and Co0.6Mn0.2Zn0.2Fe2O4 (X3) were synthesized by the coprecipitation method. The nanoparticles were characterized by XRD, Raman, VSM, XPS, and TEM. The magnetic hyperthermia efficiency (MH) was determined for PEG-coated nanoparticles using an alternating magnetic field (AMF). X2 nanoparticles displayed the highest saturation magnetization and specific absorption rate (SAR) value of 245.2 W/g for 2 mg/mL in a water medium. Based on these properties, X2 nanoparticles were further evaluated for antiproliferative activity against HCT116 cells at an AMF of 495.25 kHz frequency and 350 G strength, using MTT, colony formation, wound healing assays, and flow cytometry analysis for determining the cell viability, clonogenic property, cell migration ability, and cell death of HCT116 cells upon AMF treatment in HCT116 cells, respectively. We observed a significant inhibition of cell viability (2% for untreated control vs. 50% for AMF), colony-forming ability (530 cells/colony for untreated control vs. 220 cells/colony for AMF), abrogation of cell migration (100% wound closure for untreated control vs. 5% wound closure for AMF), and induction of apoptosis-mediated cell death (7.5% for untreated control vs. 24.7% for AMF) of HCT116 cells with respect to untreated control cells after AMF treatment. Collectively, these results demonstrated that the PEG-coated (CoMnZn-Fe2O4) mixed ferrite nanoparticles upon treatment with AMF induced a significant antiproliferative effect on HCT116 cells compared with the untreated cells, indicating the promising antiproliferative potential of the Co0.4Mn0.4Zn0.2Fe2O4 nanoparticles for targeting colorectal cancer cells. Additionally, these results provide appealing evidence that ferrite-based nanoparticles using MH could act as potential anticancer agents and need further evaluation in preclinical models in future studies against colorectal and other cancers.en_US
dc.description.sponsorshipUniversity of Sharjah and Sharjah Research Academy for the Collaborative Research; Research Institute of Medical and Health Sciences; Sharjah Institute of Medical Research (SIMR) in the University of Sharjahen_US
dc.description.sponsorshipThe authors also thank the Research Institute of Medical and Health Sciences (RIMHS) and Sharjah Institute of Medical Research (SIMR) in the University of Sharjah. We also thank Manju Nidagodu Jayakumar for his help in the flow cytometric analysis. I.A. Al-Omari would like to thank Abdul Rahman Al-Nabhani from the Electron Microscopy Unit in the college of Medicine and Health Sciences, SQU for helping with TEM measurements.en_US
dc.identifier.doi10.3390/ph17030327
dc.identifier.issn1424-8247
dc.identifier.issue3en_US
dc.identifier.pmid38543113en_US
dc.identifier.urihttps://doi.org10.3390/ph17030327
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5261
dc.identifier.volume17en_US
dc.identifier.wosWOS:001193572600001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofPharmaceuticalsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectHyperthermiaen_US
dc.subjectSuperparamagneticen_US
dc.subjectSpecific Absorption Rateen_US
dc.subjectApoptosisen_US
dc.subjectAntiproliferationen_US
dc.titleEvaluation of Antiproliferative Properties of CoMnZn-Fe2O4 Ferrite Nanoparticles in Colorectal Cancer Cellsen_US
dc.typeArticleen_US

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