Comparison of Adipocyte Viability After Short-Term Cryopreservation of Adipose Aspirates Through 3 Different Techniques

Basit Öğe Kaydı
dc.authoridKoçak, Polen/0000-0003-4493-1061
dc.authorwosidKoçak, Polen/AGG-7399-2022
dc.contributor.authorKocak, Polen
dc.contributor.authorUnsal, Naz
dc.contributor.authorCanikyan, Serli
dc.contributor.authorKul, Yaren
dc.contributor.authorCohen, Steven R.
dc.contributor.authorTiryaki, Tung
dc.date.accessioned2024-05-19T14:50:40Z
dc.date.available2024-05-19T14:50:40Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractBackground: Effective cryopreservation allows for the long-term storage of living cells or tissues with the possibility of later clinical applications. Unfortunately, no successful investigations on the long-term preservation of adipose aspirates for prospective autologous fat grafting have been conducted. Objectives: In this study, we aimed to compare 3 different freezing methods to preserve adipose aspirates obtained from conventional lipoplasty to determine the optimal cryopreservation technique. Methods: To determine the optimal cryopreservation technique, hematoxylin and eosin staining, MTS assay, and Annexin assay were performed on each of the 3 groups plus a fourth control group. Group 1 served as the control, and fat tissue was analyzed immediately after adipose harvesting with no cryopreservation. For experimental Group 2, 15mL of adipose aspirates were directly frozen at -80 degrees C for up to 2 weeks. For experimental Group 3, 15mL of adipose aspirates were frozen inside the adi-frosty containing 100% isopropanol and stored at -80 degrees C for up to 2 weeks. For experimental Group 4, 15mL of adipose aspirates were frozen with freezing solution containing 90% fetal bovine serum (v/v) and 10% dimethyl sulfoxide (v/v). Results: The results demonstrated that the experimental Group 3 had significantly more live adipocytes and greater cellular function of adipose aspirates than the experimental Groups 2 and 4. Conclusion: Cryopreservation with adi-frosty containing 100% isopropanol appears to be the best means of cryopreservation of fat.en_US
dc.identifier.doi10.1093/asjof/ojad026
dc.identifier.issn2631-4797
dc.identifier.pmid37180738en_US
dc.identifier.urihttps://doi.org10.1093/asjof/ojad026
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5776
dc.identifier.volume5en_US
dc.identifier.wosWOS:001133595800037en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherOxford Univ Pressen_US
dc.relation.ispartofAesthetic Surgery Journal Open Forumen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectFat Transferen_US
dc.subjectSurgical-Managementen_US
dc.subjectDimethyl-Sulfoxideen_US
dc.subjectGynecomastiaen_US
dc.subjectGraftsen_US
dc.subjectTissueen_US
dc.subjectLiposuctionen_US
dc.subjectLipectomyen_US
dc.subjectCellsen_US
dc.subjectSkinen_US
dc.titleComparison of Adipocyte Viability After Short-Term Cryopreservation of Adipose Aspirates Through 3 Different Techniquesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu