Long-term preservation of Cicer arietinum L. germplasm by in vitro propagation and cryopreservation

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dc.authoridElif Aylin Özüdoğru / 0000-0002-5871-0617
dc.authorscopusidElif Aylin Özüdoğru / 8677777800
dc.authorwosidElif Aylin Özüdoğru / AAP-8117-2020
dc.contributor.authorRuta, Claudia
dc.contributor.authorDe Mastro, Giuseppe
dc.contributor.authorTarraf, Waed
dc.contributor.authorAncona, Simona
dc.contributor.authorTagarelli, Anna
dc.contributor.authorÖzüdoğru, Elif Aylin
dc.contributor.authorLambardi, Maurizio
dc.date.accessioned2020-08-30T20:01:34Z
dc.date.available2020-08-30T20:01:34Z
dc.date.issued2020
dc.departmentİstinye Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.description.abstractChickpea (Cicer arietinum L.) is one of the most important pulse worldwide. This crop plays a significant role to maintain soil fertility through symbiotic N fixation, as well as in the Mediterranean diet for its important content of noble protein. In South Italy, particularly in the Puglia region, many traditional landraces are still cultivated in marginal areas, becoming therefore at strong risk of genetic erosion or even extinction. In vitro culture is a useful and innovative approach for the collection and the long-term preservation of threatened germplasm by means of the cryopreservation technology, as ex situ conservation strategy complementary to traditional ones. The aim of this study was to develop an efficient protocol for the multiplication and cryopreservation of two accessions of an Apulian black chickpea threatened landrace. Seeds of Apulian black chickpea were inoculated on agarized sucrose-free nutrient medium. The cotyledonary node and axillary buds were excised from the seedlings and then cultured on the same medium, supplemented with 6-benzylaminopurine and sucrose. After three subculture cycles, shoot tips from in vitro proliferated shoots were induced to rooting on IBA or used for germplasm conservation by comparing three cryopreservation techniques, i.e. PVS2-vitrification, droplet-vitrification and V-cryoplate. The results are very promising in terms of explant survival. However, the study should now progress to optimize a recovery medium, able to further improve shoot regrowth rates and plantlet formation in post-cryopreservation. © 2019, Springer Nature B.V.en_US
dc.description.sponsorship1698/2005en_US
dc.description.sponsorshipThis study was funded by REGIONAL PROJECT SaVeGraINPuglia (Reg.CE n. 1698/2005 Programma di Sviluppo rurale per la Puglia 2007/2013 Misura 214 – Azione 4 Sub azione a) “Progetti integrati per la Biodiversità”). Maurizio Lambardi gratefully thanks the “International Treaty on Plant Genetic Resources for Food and Agriculture - RGV-FAO, 2017–2019” for partially supporting the study.en_US
dc.description.sponsorshipThis research is supported by the REGIONAL PROJECT SaVeGraINPuglia (Reg.CE n. 1698/2005 Programma di Sviluppo rurale per la Puglia 2007/2013 Misura 214 – Azione 4 Sub azione a) “Progetti integrati per la Biodiversità”).en_US
dc.identifier.citationRuta, C., De Mastro, G., Tarraf, W., Ancona, S., Tagarelli, A., Ozudogru, A., & Lambardi, M. (2020). Long-term preservation of Cicer arietinum L. germplasm by in vitro propagation and cryopreservation. Genetic Resources and Crop Evolution, 67(2), 263-271.en_US
dc.identifier.doi10.1007/s10722-019-00867-6en_US
dc.identifier.endpage271en_US
dc.identifier.issn0925-9864en_US
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85076596687en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage263en_US
dc.identifier.urihttps://doi.org/10.1007/s10722-019-00867-6
dc.identifier.urihttps://hdl.handle.net/20.500.12713/291
dc.identifier.volume67en_US
dc.identifier.wosWOS:000511990300001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorÖzüdoğru, Elif Aylinen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofGenetic Resources and Crop Evolutionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApulian Landraceen_US
dc.subjectBiological Diversity Maintenanceen_US
dc.subjectChickpeaen_US
dc.subjectGermplasm Multiplicationen_US
dc.subjectIn Vitro Storageen_US
dc.titleLong-term preservation of Cicer arietinum L. germplasm by in vitro propagation and cryopreservationen_US
dc.typeArticleen_US

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