Neuroprotective Effects of Wharton Jelly Stem Cell-Derived Exosomes Developed as Nano-Drug Delivery System in 6-OHDA-Induced Neurotoxicity in 2D and 3D Neuronal Cell Line

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dc.authoridAllahverdiyev, Adil M./0000-0002-7031-5986
dc.authoridAlomary, Mohammad Nasser/0000-0001-7909-4377
dc.authoridZorba Yildiz, Asli Pinar/0000-0001-6854-9890
dc.authoridYavuz, Burcak/0000-0002-3555-4850
dc.authorwosidAllahverdiyev, Adil M./ISU-9244-2023
dc.authorwosidAlomary, Mohammad Nasser/AAH-5788-2019
dc.contributor.authorYavuz, Burcak
dc.contributor.authorYildiz, Asli Pinar Zorba
dc.contributor.authorAbamor, Emrah Sefik
dc.contributor.authorDarici, Hakan
dc.contributor.authorAllahverdiyev, Adil M.
dc.date.accessioned2024-05-19T14:46:28Z
dc.date.available2024-05-19T14:46:28Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractPurposeThe underlying mechanism of today's neurodegenerative diseases is disordered due to neuronal damage and very limited neuronal regeneration. Formulations that can be developed against the factors that cause damage must have a neuroprotective effect. Particularly, formulations that can be developed in nanoscales may play a key role in central nervous system diseases. This article aimed to investigate the neuroprotective activity of dopamine-loaded exosomes in which exosomes derived from Wharton jelly stem cells (WJ-MSCs) are used as carrier systems in 2D and 3D cell cultures induced in cell damage.Materials and MethodsThis study created 2D and 3D cell cultures with a bioprinter in the dopaminergic neuroblastoma cell line (SH-SY5Y). Cell lines were damaged with 6-OHDA. The neuroprotective effect of dopamine-loaded exosomes was investigated by Live&Dead analysis and immunostaining.ResultsThe findings showed that dopamine-loaded exosomes protected against 6-OHDA-induced neurotoxicity in both cultures. The increase in the number of viable cells in the 6-OHDA administered groups proved the neuroprotective effect.ConclusionWe predict that the carrier systems developed with exosomes obtained from Wharton jelly stem cells will be a light of hope in the treatment of neurodegenerative diseases, considering both their size and their contribution to regeneration.Lay SummaryEspecially in neurodegenerative diseases, it is very important to be able to protect and regenerate neurons. Wharton jelly stem cell-based exosomes are known to have many properties, including other wound healing activities. In addition, considering both the structure and size of exosomes, its development as a drug delivery system is promising.en_US
dc.description.sponsorshipYildiz Technical University Scientific Research Projects Coordination Department (YTU BAP) [TSA-2021-4713]en_US
dc.description.sponsorshipThis work was supported by the Yildiz Technical University Scientific Research Projects Coordination Department (YTU BAP, Project Number: TSA-2021-4713), respectivelyen_US
dc.identifier.doi10.1007/s40883-023-00322-0
dc.identifier.issn2364-4133
dc.identifier.issn2364-4141
dc.identifier.scopus2-s2.0-85176791243en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org10.1007/s40883-023-00322-0
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5528
dc.identifier.wosWOS:001102567700001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofRegenerative Engineering and Translational Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectRegenerative Medicineen_US
dc.subjectExosomeen_US
dc.subjectWharton Jelly Stem Cellen_US
dc.subjectDrug Delivery Systemen_US
dc.subject3d Cell Cultureen_US
dc.subjectNeuroprotective Effectsen_US
dc.titleNeuroprotective Effects of Wharton Jelly Stem Cell-Derived Exosomes Developed as Nano-Drug Delivery System in 6-OHDA-Induced Neurotoxicity in 2D and 3D Neuronal Cell Lineen_US
dc.typeArticleen_US

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