Metal Oxide Nanoparticles Exhibit Anti-Acanthamoeba castellanii Properties by Inducing Necrotic Cell Death

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dc.authorscopusidRuqaiyyah Siddiqui / 18635417000
dc.authorscopusidNaveed Ahmed Khan / 7401797675
dc.authorwosidRuqaiyyah Siddiqui / FVT-1496-2022
dc.authorwosidNaveed Ahmed Khan / FXL-5617-2022
dc.contributor.authorAhmed, Usman
dc.contributor.authorGew, Lai Ti
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.contributor.authorKhan, Naveed Ahmed
dc.contributor.authorAlharbi, Ahmad M.
dc.contributor.authorAlhazmi, Ayman
dc.contributor.authorAnwar, Ayaz
dc.date.accessioned2025-04-18T10:28:22Z
dc.date.available2025-04-18T10:28:22Z
dc.date.issued2024
dc.departmentİstinye Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü
dc.description.abstractPurpose: The treatment of amoebic infections is often problematic, largely due to delayed diagnosis, amoebae transformation into resistant cyst form, and lack of availability of effective chemotherapeutic agents. Herein, we determined anti-Acanthamoeba castellanii properties of three metal oxide nanoparticles (TiO2, ZrO2, and Al2O3). Methods: Amoebicidal assays were performed to determine whether metal oxide nanoparticles inhibit amoebae viability. Encystation assays were performed to test whether metal oxide nanoparticles inhibit cyst formation. By measuring lactate dehydrogenase release, cytotoxicity assays were performed to determine human cell damage. Hoechst 33342/PI staining was performed to determine programmed cell death (apoptosis) and necrosis in A. castellanii. Results: TiO2-NPs significantly inhibited amoebae viability as observed through amoebicidal assays, as well as inhibited their phenotypic transformation as evident using encystation assays, and showed limited human cell damage as observed by measuring lactate dehydrogenase assays. Furthermore, TiO2-NPs altered parasite membranes and resulted in necrotic cell death as determined using double staining cell death assays with Hoechst33342/Propidium iodide (PI) observed through chromatin condensation. These findings suggest that TiO2-NPs offers a potential viable avenue in the rationale development of therapeutic interventions against Acanthamoeba infections. © The Author(s), under exclusive licence to Springer Nature Switzerland AG 2024.
dc.description.sponsorshipThis research work was supported by Sunway University, Malaysia (Individual research grant, GRTIN-IRG-10-2022). This research was funded by Taif University, Taif, Saudi Arabia (TU-DSPP-2024-147). Ruqaiyyah Siddiqui and Naveed Ahmed Khan are supported by the Air Force Office of Scientific Research (AFOSR), USA. Funding text 2 We are grateful to Sunway University for their invaluable support and sponsorship of this research endeavor. The authors extend their appreciation to Taif University, Saudi Arabia, for supporting this work through project number ( TU-DSPP-2024-147).
dc.identifier.citationAhmed, U., Gew, L. T., Siddiqui, R., Khan, N. A., Alharbi, A. M., Alhazmi, A., & Anwar, A. (2024). Metal Oxide Nanoparticles Exhibit Anti-Acanthamoeba castellanii Properties by Inducing Necrotic Cell Death. Acta parasitologica, 69(3), 1717-1723.
dc.identifier.doi10.1007/s11686-024-00891-2
dc.identifier.endpage1723
dc.identifier.issn12302821
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85201542757
dc.identifier.scopusqualityQ3
dc.identifier.startpage1717
dc.identifier.urihttp://dx.doi.org/10.1007/s11686-024-00891-2
dc.identifier.urihttps://hdl.handle.net/20.500.12713/7081
dc.identifier.volume69
dc.identifier.wosWOS:001292868200002
dc.identifier.wosqualityQ2
dc.indekslendigikaynakScopus
dc.indekslendigikaynakWeb of Science
dc.institutionauthorSiddiqui, Ruqaiyyah
dc.institutionauthorKhan, Naveed Ahmed
dc.institutionauthoridRuqaiyyah Siddiqui / 0000-0001-9646-6208
dc.institutionauthoridNaveed Ahmed Khan / 0000-0001-7667-8553
dc.language.isoen
dc.publisherSpringer Science and Business Media Deutschland GmbH
dc.relation.ispartofActa Parasitologica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectA. castellanii
dc.subjectAnti-Amoebic
dc.subjectIn Vitro
dc.subjectMetal Oxide-Nanoparticles
dc.subjectNecrotic Cell Death
dc.titleMetal Oxide Nanoparticles Exhibit Anti-Acanthamoeba castellanii Properties by Inducing Necrotic Cell Death
dc.typeArticle

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