Nonapoptotic cell death induced by Hypericum species on cancer cells
Yükleniyor...
Dosyalar
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objectives. There are approximately 400 Hypericum species that grow naturally in different geographic originsof the world. Those species have been used in different folk medicines and screened for their biological activityincluding cancer. Our country is an important place for Hypericum species which are known as “kantaron,binbirdelik otu, kan otu, kılıç otu, yaraotu, kuzukıran”. We therefore evaluated the possible cytotoxic/apoptoticactivities, total phenolic content and antioxidant capacity of the crude methanol extracts Hypericumadenotrichum Spach. and Hypericum olympicum L. which are still used in Turkish folk medicine. Methods.The total phenolic content and antioxidant capacity were determined by Folin-Ciocalteu and ABTS methods.Anti-growth effects were screened in human hepatoma (Hep3B) and rat glioma (C6) cell lines by the MTTand ATP viability assays. The cell death mode (apoptosis/necrosis) was investigated by fluorescence imagingand the level of caspase-cleaved cytokeratin 18 (M30), active caspase-3 and cleaved PARP (poly (ADP-ribose)polymerase). Results. The results indicate that the crude methanol extracts of Hypericum olympicum L. andHypericum adenotrichum have both anti-growth/cytotoxic activities on these cells in a dose dependent manner.Conclusion. These extracts clearly induced non-apoptotic cell death in human hepatoma (Hep3B) and ratglioma (C6) cell lines
Açıklama
Anahtar Kelimeler
Hypericum Olympicum, Hypericum Adenotrichum, Cell Death, Necrosis
Kaynak
The European Research Journal
WoS Q Değeri
Scopus Q Değeri
Cilt
3
Sayı
1
Künye
ARI F,AZTOPAL N,ERKİSA M,ÇELİKLER S,ŞAHİN S,ULUKAYA E (2017). Nonapoptotic cell death induced by Hypericum species on cancer cells. The European Research Journal, 3(1), 1 - 10.
