Cytotoxic effects of n-(4-chlorophenyl)-1h-ındole-2-carboxamide on bone cancer cells
Yükleniyor...
Dosyalar
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
INTRODUCTION:Osteosarcoma is a common tumor of high malignancy, usually derived from mesenchymal tissues. The lack of response to chemotherapeutic treatments indicates the importance of investigating new therapeutic methods. Indole, which is known to inhibit proliferation on cancer cells, is new synthesized as a derivative of this molecule of N-(4-chlorophenyl) -lH-indole-2-carboxamide, has an effect on osteosarcoma cell line. We evaluated the cytotoxic effects of N-(4-chlorophenyl) -1H-indole-2-carboxamide by verification of pharmacological doses of imatinib mesylate based on time on osteosarcoma cells.METHODS:In the present study, the effects of N-(4-chlorophenyl)-1H-indole-2-carboxamide and Imatinib mesylate doses on cell viability of osteosarcoma Saos-2 cells were conducted using MTT assay. Ezrin expression levels was examined by immunofluorescence staining.RESULTS:The results of the antiproliferative activity showed that N-(4-chlorophenyl) -1 H-indole-2-carboxamide inhibited cell proliferation of Saos-2 cells in a dose-and time-dependent manner. We found that even lower doses of N-(4-chlorophenyl) -1H-indole-2-carboxamide was effective on osteosarcoma cell lines when we compared with imatinib mesylate pharmacological doses.DISCUSSION AND CONCLUSION:In this study, for the first time the newly synthesized indole derivative molecule, N-(4-chlorophenyl) -1 H-indole-2-carboxamide was evaluated for resistance in vitro Saos-2 cell line. All these results showed that N-(4-Chlorophenyl) -1 H-Indole-2-Carboxamide can be developed as a potential therapeutic agent for inhibiting osteosarcoma growth and metastasis
Açıklama
Anahtar Kelimeler
Osteosarcoma, N-(4-Chlorophenyl)-1H-Indole-2-Carboxamide, Cell Proliferation, Reactive Oxygen Species, Thyrosine Kinases
Kaynak
ACTA ONCOLOGICA TURCICA
WoS Q Değeri
Scopus Q Değeri
Cilt
53
Sayı
1
Künye
TAŞDEMİR M,ÖZGÜRSES M. E,DARICI H,ORDUERİ N. E (2020). Cytotoxic Effects Of N-(4-Chlorophenyl)-1H-Indole-2-Carboxamide On Bone Cancer Cells. ACTA ONCOLOGICA TURCICA, 53(1), 173 - 180. Doi: 10.5505/aot.2020.20591
