Determination of protein amount in nanosized synthetic liposomes by Surface Enhanced Raman Spectroscopy (SERS)
Yükleniyor...
Tarih
9
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Çanakkale Onsekiz Mart University
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Accurate characterization of synthetic liposomes is essential since they give information about the vesic ular structures in bodily fluids such as extracellular vesicles. The characterization tasks are generally the determina tion of the sizes of the liposomes and the profiling of the liposomes' content. Optical tweezers and Surface Enhanced
Raman Spectroscopy (SERS) were used to profile the nanosized liposomes. The size distribution of the trapped lip osomes (140 nm on average) was found by using Einstein's Brownian motion equation, consistent with the size dis tribution obtained from dynamic light scattering measurements. Besides, Gramicidin-encapsulated liposomes were
measured using SERS, and statistically significant differentiation was found in Raman intensities between liposome
populations with altering concentrations of proteins. This study uniquely measured size distributions of nano-sized
liposomes with conventional optical tweezers (without plasmonics) and determined the chemical differences between
empty and protein encapsulated liposomes with high accuracy using Raman spectroscopy.
Açıklama
Anahtar Kelimeler
Liposome, Optical Trapping, Surface Enhanced Raman Spectroscopy, Gramicidin, Protein Determination
Kaynak
Journal of Advanced Research in Natural and Applied Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
9
Sayı
4
Künye
Parlatan, Ş. Determination of Protein Amount in Nanosized Synthetic Liposomes by Surface Effect Raman Spectroscopy (SERS). Journal of Advanced Research in Natural and Applied Sciences, 9(4), 912-922.
