Investigation of preconditioning and the protective effects of nicotinamide against cerebral ischemia-reperfusion injury in rats

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dc.authorscopusidRuhat Arslan / 59295960800
dc.authorwosidRuhat Arslan / JZM-7258-2024
dc.contributor.authorArslan, Ruhat
dc.contributor.authorDoğanay, Songül
dc.contributor.authorBudak, Özcan
dc.date.accessioned2025-04-18T10:45:27Z
dc.date.available2025-04-18T10:45:27Z
dc.date.issued2024
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü
dc.description.abstractThis study investigated the antioxidant and neuroprotective effects of nicotinamide combined with ischemic preconditioning against cerebral ischemia reperfusion (CIR) injury. Thirty-five Wistar albino male rats were randomly divided into five groups: sham, preconditioned ischemia/reperfusion (IP+IR), ischemia/reperfusion (IR), preconditioned ischemia/reperfusion + nicotinamide (IP+IR+N), and ischemia/reperfusion + nicotinamide (IR+N). CIR was achieved with bilateral common carotid artery occlusion. IP+IR and IP+IR+N groups 30 min before ischemia; Three cycles of 10 sec ischemia/30 sec reperfusion followed by 20 min IR were applied. The IP+IR+N and IR+N groups received 500 mg/kg nicotinamide intraperitoneally. After 24 h of reperfusion, a neurological evaluation was performed and vertıcal pole test. Biochemically, malondialdehyde (MDA), glutathione (GSH) levels and catalase (CAT) activity were measured in blood and brain tissue samples. Rates of red neurons, sateliosis and spongiosis were determined histopathologically in the prefrontal cortex areas. After CIR, MDA levels increased significantly in serum and brain tissue in the IR group compared to the sham group, while GSH and CAT activity decreased in the brain tissue (p < 0.05). MDA levels in the tissues were found significantly decreased in the IR+N group compared to the IR group (p < 0.05). Administration of nicotinamide together with IP significantly decreased MDA levels in brain tissue and increased GSH and CAT activity (p < 0.05). Compared to the IR group, the morphological and neurological damage in the prefrontal cortex areas decreased in the IP+IR, IP+IR+N, and IR+N groups (p < 0.05). In addition, red neuron, sateliosis and spongiosis rates increased significantly in the IR group compared to the Sham, IP+IR+N, IR+N groups (p < 0.001 for all). In neurological evaluation, while the neurological score increased and the time on the vertical pole decreased significantly in the IR group, preconditioning, and nicotinamide groups reversed (p < 0.05). The study's results show that nicotinamide administration with ischemic preconditioning alleviates cerebral ischemia/reperfusion injury. © 2024 Elsevier B.V.
dc.identifier.citationArslan, R., Doganay, S., Budak, O., & Bahtiyar, N. (2024). Investigation of preconditioning and the protective effects of nicotinamide against cerebral ischemia-reperfusion injury in rats. Neuroscience Letters, 840, 137949.
dc.identifier.doi10.1016/j.neulet.2024.137949
dc.identifier.issn03043940
dc.identifier.scopus2-s2.0-85201863923
dc.identifier.scopusqualityQ2
dc.identifier.urihttp://dx.doi.org/10.1016/j.neulet.2024.137949
dc.identifier.urihttps://hdl.handle.net/20.500.12713/7178
dc.identifier.volume840
dc.identifier.wosWOS:001302631100001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakScopus
dc.indekslendigikaynakWeb of Science
dc.institutionauthorArslan, Ruhat
dc.institutionauthoridRuhat Arslan / 0000-0002-5611-2656
dc.language.isoen
dc.publisherElsevier Ireland Ltd
dc.relation.ispartofNeuroscience Letters
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCerebral İschemia/Reperfusion
dc.subjectNicotinamide
dc.subjectOxidative Stress
dc.subjectPreconditioning
dc.titleInvestigation of preconditioning and the protective effects of nicotinamide against cerebral ischemia-reperfusion injury in rats
dc.typeArticle

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