Pioneering in vitro studies for callus formation of colchicum chalcedonicum azn.

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dc.authoridElif Karlık / 0000-0003-0669-2725en_US
dc.authorscopusidElif Karlık / 56926577900
dc.authorwosidElif Karlık / N-6348-2018
dc.contributor.authorKarlık, Elif
dc.contributor.authorDeğer, Meltem
dc.contributor.authorÜzen, Erdal
dc.contributor.authorGözükırmızı, Nermin
dc.date.accessioned2020-11-17T08:07:53Z
dc.date.available2020-11-17T08:07:53Z
dc.date.issued2020en_US
dc.departmentİstinye Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.description.abstractColchicum calcedonicum Azn is one of the endemic species distributed in Turkey, where many endemic plant species occur. It has long-oval shaped corm under the soil, and usually 3-4 leaves on it. In vitro production of endemic species using callus culture has become promising study for conservation. The aim of this study is to generate an efficient callus protocol for in vitro production of C. chalcedonicum. To sterilize the explants, 0.25% (w/v) mercuric chloride (HgCl2) was used for 20 min. In addition to mercuric chloride, surface sterilization was conducted by using 6.5% NaCl with Tween 80 for 30 min. We used 19 different mediums and the primary callus formation was obtained in Murashige & Skoog's basal medium (MS) supplemented with 2,4-D (2 mg L-1), 2IP (0.5 mg L-1), 3% sucrose and 0.05% active carbon. Our study demonstrated the active carbon usage was effective for the primary callus formation. This study is the first report for primary callus formation of C. chalcedonicum. However, our work is a pioneering study to improve callus formation protocol system for in vitro conservation of endemic species C. chalcedonicum.en_US
dc.identifier.citationKARLIK, E., DEĞER, M., Erdal, Ü. Z. E. N., & GOZUKİRMİZİ, N. PIONEERING in vitro STUDIES FOR CALLUS FORMATION OF Colchicum chalcedonicum Azn. Trakya University Journal of Natural Sciences, 21(2), 131-137.en_US
dc.identifier.doi10.23902/trkjnat.776974en_US
dc.identifier.endpage137en_US
dc.identifier.isbn2147-0294
dc.identifier.issue2en_US
dc.identifier.startpage131en_US
dc.identifier.trdizinid429503en_US
dc.identifier.urihttps://doi.org/10.23902/trkjnat.776974
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1196
dc.identifier.volume21en_US
dc.identifier.wosWOS:000582931800007en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorKarlık, Elif
dc.language.isoenen_US
dc.publisherTRAKYA UNIV BALKAN YERLESESI ENSTITULER BINASIen_US
dc.relation.ispartofTRAKYA UNIVERSITY JOURNAL OF NATURAL SCIENCESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectChemical-Constituentsen_US
dc.subjectDidderent Partsen_US
dc.subjectResistanceen_US
dc.subjectAlkaloidsen_US
dc.subjectPlanten_US
dc.titlePioneering in vitro studies for callus formation of colchicum chalcedonicum azn.en_US
dc.typeArticleen_US

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