Design, Synthesis, and Comparison of PLA-PEG-PLA and PEG-PLA-PEG Copolymers for Curcumin Delivery to Cancer Cells

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dc.authoridHeidari, Reza/0000-0003-2985-8396
dc.authoridgomari, mohammad mahmoudi/0000-0003-4143-2208
dc.authoridUversky, Vladimir N./0000-0002-4037-5857
dc.authoridAbdouss, Majid/0000-0003-2305-7985
dc.authoridMahmoudi Gomari, Mohammad/0000-0003-4143-2208
dc.authoridRostami, Neda/0009-0009-4547-3219
dc.authoridAminoroaya, Alireza/0000-0002-8716-642X
dc.authorwosidHeidari, Reza/JQW-7989-2023
dc.authorwosidgomari, mohammad mahmoudi/T-2991-2018
dc.authorwosidUversky, Vladimir N./F-4515-2011
dc.authorwosidAbdouss, Majid/E-2016-2017
dc.authorwosidMahmoudi Gomari, Mohammad/Y-6938-2018
dc.contributor.authorRostami, Neda
dc.contributor.authorFaridghiasi, Farzaneh
dc.contributor.authorGhebleh, Aida
dc.contributor.authorNoei, Hadi
dc.contributor.authorSamadzadeh, Meisam
dc.contributor.authorGomari, Mohammad Mahmoudi
dc.contributor.authorTajiki, Alireza
dc.date.accessioned2024-05-19T14:39:37Z
dc.date.available2024-05-19T14:39:37Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractCurcumin (CUR) has potent anticancer activities, and its bioformulations, including biodegradable polymers, are increasingly able to improve CUR's solubility, stability, and delivery to cancer cells. In this study, copolymers comprising poly (L-lactide)-poly (ethylene glycol)-poly (L-lactide) (PLA-PEG-PLA) and poly (ethylene glycol)-poly (L-lactide)-poly (ethylene glycol) (PEG-PLA-PEG) were designed and synthesized to assess and compare their CUR-delivery capacity and inhibitory potency on MCF-7 breast cancer cells. Molecular dynamics simulations and free energy analysis indicated that PLA-PEG-PLA has a higher propensity to interact with the cell membrane and more negative free energy, suggesting it is the better carrier for cell membrane penetration. To characterize the copolymer synthesis, Fourier transform-infrared (FT-IR) and proton nuclear magnetic resonance (H-1-NMR) were employed, copolymer size was measured using dynamic light scattering (DLS), and their surface charge was determined by zeta potential analysis. Characterization indicated that the ring-opening polymerization (ROP) reaction was optimal for synthesizing high-quality polymers. Microspheres comprising the copolymers were then synthesized successfully. Of the two formulations, PLA-PEG-PLA experimentally exhibited better results, with an initial burst release of 17.5%, followed by a slow, constant release of the encapsulated drug up to 80%. PLA-PEG-PLA-CUR showed a significant increase in cell death in MCF-7 cancer cells (IC50 = 23.01 & PLUSMN; 0.85 & mu;M) based on the MTT assay. These data were consistent with gene expression studies of Bax, Bcl2, and hTERT, which showed that PLA-PEG-PLA-CUR induced apoptosis more efficiently in these cells. Through the integration of nano-informatics and in vitro approaches, our study determined that PLA-PEG-PLA-CUR is an optimal system for delivering curcumin to inhibit cancer cells.en_US
dc.identifier.doi10.3390/polym15143133
dc.identifier.issn2073-4360
dc.identifier.issue14en_US
dc.identifier.pmid37514522en_US
dc.identifier.urihttps://doi.org10.3390/polym15143133
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4815
dc.identifier.volume15en_US
dc.identifier.wosWOS:001036738800001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofPolymersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectBreast Canceren_US
dc.subjectCurcuminen_US
dc.subjectCopolymeren_US
dc.subjectDrug Deliveryen_US
dc.subjectNano-Informaticsen_US
dc.subjectBiomaterialsen_US
dc.subjectPegen_US
dc.subjectPlaen_US
dc.titleDesign, Synthesis, and Comparison of PLA-PEG-PLA and PEG-PLA-PEG Copolymers for Curcumin Delivery to Cancer Cellsen_US
dc.typeArticleen_US

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