Glioblastoma stem cells and comparison of isolation methods

Basit Öğe Kaydı
dc.authoridZehragül Ergül / 0000-0002-1466-5068
dc.authorscopusidZehragül Ergül / 57204559825
dc.authorwosidZehragül Ergül / EUM-6344-2022
dc.contributor.authorDundar, Tolga Turan
dc.contributor.authorHatiboğlu, Mustafa Aziz
dc.contributor.authorErgül, Zehragül
dc.contributor.authorSeyithanoglu, Mehmet Hakan
dc.contributor.authorSozen, Elif
dc.contributor.authorTuzgen, Saffet
dc.contributor.authorKaynar, Mehmet Yasar
dc.contributor.authorKaraöz, Erdal
dc.date.accessioned2020-09-22T12:59:41Z
dc.date.available2020-09-22T12:59:41Z
dc.date.issued2019en_US
dc.departmentİstinye Üniversitesien_US
dc.description.abstractBackground: Glioblastoma (GBM) is the most aggressive and the most common primary brain tumor. Over the last few years, studies have identified many genetical and phenotypical molecular situations for developing new treatment modalities in patients with GBM. Nevertheless, main problem for the GBM is radio-chemotherapy resistance and relapse after the surgery. The identification of glioma stem cells and microenvironmental influences has created a paradigm shift in targets of therapy. Current studies have shown that glioma stem cell is responsible for aggressiveness, recurrence and resistance to therapy of GBM. GBM stem cell isolated from human GBM multiforme fresh tissue samples is important both for curative therapeutic options and personalized targeted therapy. The purpose of this study was to determine the most suitable isolation method of GBM stem cells (GSCs). Methods: Tumor tissue sample was obtained during the surgical resection of lesion in patients with the diagnosis of GBM. Tumor stem cell isolation from tissue was performed in three different ways: 1) GBM cell isolation with trypsin; 2) GBM cell isolation with brain tumor dissociation Kit (BTD Kit); and 3) GBM cell isolation with tumor dissociation enzyme (TDE). Results: We showed that GSCs were isolated from tumor specimen using flow cytometry and immunofluorescence staining. Our study showed that isolation with BTD Kit is the most suitable method to isolate GBM tissue-derived glial tumor stem cells. Conclusions: The development of alternative personalized therapies targeting brain tumor stem cell is urgently needed. It is important to understand the fundamental mechanisms of driving stem cells. If their life cycle mechanisms can be identified, we can control the growth of GBM. Keywords: Cancer stem cell; Glioblastoma; Targeted therapy.en_US
dc.identifier.citationDundar, T. T., Hatiboglu, M. A., Ergul, Z., Seyithanoglu, M. H., Sozen, E., Tuzgen, S., ... & Karaoz, E. (2019). Glioblastoma Stem Cells and Comparison of Isolation Methods. Journal of Clinical Medicine Research, 11(6), 415.en_US
dc.identifier.doi10.14740/jocmr3781en_US
dc.identifier.endpage421en_US
dc.identifier.issue11en_US
dc.identifier.pmid31143308en_US
dc.identifier.startpage415en_US
dc.identifier.urihttps://doi.org/10.14740/jocmr3781
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1069
dc.identifier.volume6en_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorErgül, Zehragül
dc.language.isoenen_US
dc.relation.ispartofJ Clin Med Resen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGlioblastomaen_US
dc.subjectCancer Stem Cellen_US
dc.subjectTargeted Therapyen_US
dc.titleGlioblastoma stem cells and comparison of isolation methodsen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
zehragul.pdf
Boyut:
2.01 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

PubMed İndeksli Yayınlar Koleksiyonu