Immunoreactivity of Kir3.1, muscarinic receptors 2 and 3 on the brainstem, vagus nerve and heart tissue under experimental demyelination

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dc.authoridAKYUZ, Enes/0000-0002-3942-2097
dc.authoridUçar, Sümeyye/0000-0003-3378-3745
dc.authorwosidOkan, Aslı/I-8771-2017
dc.authorwosidAKYUZ, Enes/W-7801-2018
dc.authorwosidUçar, Sümeyye/AAL-8741-2021
dc.contributor.authorAkyuz, Enes
dc.contributor.authorDoganyigit, Zueleyha
dc.contributor.authorOkan, Asli
dc.contributor.authorYilmaz, Seher
dc.contributor.authorUcar, Sumeyye
dc.contributor.authorAkin, Ali Tugrul
dc.date.accessioned2024-05-19T14:40:36Z
dc.date.available2024-05-19T14:40:36Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractAims: Demyelination affects the propogation of neuronal action potential by slowing down the progression. This process results in a neuro-impairment like Multiple Sclerosis (MS). Evidence show that MS also contributes to involvement of the autonomic system. In the molecular approach to this involvement, we aimed to observe muscarinic ACh receptor 2-3 (mAChR2-3), and inwardly rectifying potassium channel 3.1 (Kir3.1) immunore-activities on the brainstem, vagus nerve, and heart under cuprizone model.Main methods: Wistar albino rats were randomly divided into 8 groups; duplicating 4 groups as male and female: control groups (n = 3 +3), Cuprizone groups (n = 12 +12), sham groups (n = 4 +4), and carboxy-methyl-cellulose groups (n = 3 +3). Cuprizone-fed rats underwent demyelination via Luxol fast blue (LFB) staining of the hippocampus (Gyrus dentatus and Cornu Ammonis) and cortex. Immunohistochemistry analysis followed to the pathologic measurement of the brainstem, vagus nerve, and heart for mAChR2, mAChR3 and Kir3.1 proteinsKey findings: A significant demyelination was observed in the hippocampus and cortex tissues of rats in the female and male cuprizone groups. Myelin basic protein immunoreactivity demonstrated that cuprizone groups, in both males and females, had down-regulation in the hippocampus and cortex areas. The weights of the cuprizone-fed rats significantly decreased over six weeks. Dilated blood vessels and neuronal degeneration were severe in the hippocampus and cortex of the cuprizone groups. In the female cuprizone group, expression of mAChR2 and mAChR2 was significantly increased in the brainstem, atrium/ventricle of heart, and left/right sections of vagus nerve. Kir3.1 channels were also up-regulated in the left vagus nerve and heart sections of the female cuprizone groupSignificance: Especially in our data where female-based significant results were obtained reveal that demyelin-ation may lead to significant mAChR2, mAChR3 and Kir3.1 changes in brainstem, vagus nerve, and heart. A high immunoreactive response to demyelination at cholinergic centers may be a new targeten_US
dc.description.sponsorshipTUBITAK institution [121S848]en_US
dc.description.sponsorshipThis work was supported by the TUBITAK institution with the project number 121S848.en_US
dc.identifier.doi10.1016/j.brainresbull.2023.03.009
dc.identifier.endpage30en_US
dc.identifier.issn0361-9230
dc.identifier.issn1873-2747
dc.identifier.pmid36967090en_US
dc.identifier.startpage13en_US
dc.identifier.urihttps://doi.org10.1016/j.brainresbull.2023.03.009
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4987
dc.identifier.volume197en_US
dc.identifier.wosWOS:000972407600001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofBrain Research Bulletinen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectCuprizoneen_US
dc.subjectMultiple Sclerosisen_US
dc.subjectPotassium Channelen_US
dc.subjectMuscarinic Acetylcholine Receptoren_US
dc.titleImmunoreactivity of Kir3.1, muscarinic receptors 2 and 3 on the brainstem, vagus nerve and heart tissue under experimental demyelinationen_US
dc.typeArticleen_US

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