The Effect of Hybrosome (Umbilical Cord Blood Exosome-Liposome Hybrid Vesicles) on Human Dermal Cells In Vitro

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dc.authoridKoçak, Polen/0000-0003-4493-1061
dc.authoridWesson, Jordan/0000-0002-0059-8079
dc.authorwosidKoçak, Polen/AGG-7399-2022
dc.contributor.authorKocak, Polen
dc.contributor.authorUnsal, Naz
dc.contributor.authorCanikyan, Serli
dc.contributor.authorKul, Yaren
dc.contributor.authorCohen, Steven R.
dc.contributor.authorTiryaki, Tung
dc.contributor.authorDuncan, Diane
dc.date.accessioned2024-05-19T14:50:41Z
dc.date.available2024-05-19T14:50:41Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractBackground: Wound healing is a process that involves multiple physiological steps, and despite the availability of various wound treatment methods, their effectiveness is still limited due to several factors, including cost, efficiency, patient-specific requirements, and side effects. In recent years, nanovesicles called exosomes have gained increasing attention as a potential wound care solution due to their unique cargo components which enable cell-to-cell communication and regulate various biological processes. Umbilical cord blood plasma (UCBP) exosomes have shown promise in triggering beneficial signaling pathways that aid in cell proliferation and wound healing. However, there is still very limited information about the wound-healing effect of UCBP exosomes in the literature. Objectives: The primary objective of this study was to investigate the hybrosome technology generated with calf UCBP-derived exosome-liposome combination. Methods: The authors developed hybrosome technology by fusing cord blood exosome membranes with liposomes. Nanovesicle characterization, cell proliferation assay, wound-healing scratch assay, immunohistochemistry analysis, anti-inflammation assay, real-time polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay, and cellular uptake studies were performed using the novel hybrid exosomes. Results: Experimental results showed that hybrosome increases cell proliferation and migration by 40% to 50%, depending on the dose, and induces an anti-inflammatory effect on different cell lines as well as increased wound healing-related gene expression levels in dermal cells in vitro. All in all, this research widens the scope of wound-healing therapeutics to the novel hybrosome technology. Conclusions: UCBP-based applications have the potential for wound treatments and are promising in the development of novel therapies. This study shows that hybrosomes have outstanding abilities in wound healing using in vitro approaches.en_US
dc.identifier.doi10.1093/asjof/ojad039
dc.identifier.issn2631-4797
dc.identifier.pmid37214180en_US
dc.identifier.urihttps://doi.org10.1093/asjof/ojad039
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5777
dc.identifier.volume5en_US
dc.identifier.wosWOS:001133595800049en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherOxford Univ Pressen_US
dc.relation.ispartofAesthetic Surgery Journal Open Forumen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectNeuromuscular Electrical-Stimulationen_US
dc.subjectMuscle Stimulationen_US
dc.subjectAngiogenesisen_US
dc.subjectStrengthen_US
dc.titleThe Effect of Hybrosome (Umbilical Cord Blood Exosome-Liposome Hybrid Vesicles) on Human Dermal Cells In Vitroen_US
dc.typeArticleen_US

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