Manual versus automated volume reduction of cord blood

Basit Öğe Kaydı
dc.authoridPınar Yurdakul Mesutoğlu / 0000-0002-0043-7187
dc.authorscopusidPınar Yurdakul Mesutoğlu / 57213609711
dc.authorwosidPınar Yurdakul Mesutoğlu / AAY-4551-2021en_US
dc.contributor.authorYurdakul Mesutoğlu, Pınar
dc.contributor.authorGençer Öncül, Emine Begüm
dc.contributor.authorAkın, Hasan Yalım
dc.contributor.authorBeksaç, Meral
dc.date.accessioned2022-07-18T11:34:08Z
dc.date.available2022-07-18T11:34:08Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractObjectives: All cord blood banks all over the world follow a common procedure, concentrating progenitor cells by volume reduction, with the main purpose of optimizing the use of storage space. The main objective of this study was to compare CD34 and total nucleated cell recovery rates and red blood cell depletion efficiencies following cord blood processing using automated Sepax or manual CellEffic cord blood processing systems. Methods: Nine cord blood units with high volumes were divided into 2 equal fractions and processed with CellEffic cord blood and Sepax. Total nucleated cell, mononuclear cells, CD34+, red blood cell and total nucleated cell viability, and clonogenic assays were performed, and recovery rates were calculated on pre- and post-process cord blood units and after freeze/thaw process. In the comparison group, post-thaw differential cell counting was also performed. Results: Our results showed that post-process total nucleated cell viability with CellEffic cord blood was slightly higher than Sepax, whereas Sepax post-process total nucleated cell/ mononuclear cell values were superior to CellEffic cord blood. Postthaw red blood cell depletion was better for CellEffic cord blood. Post-thaw Sepax colony-forming unit counts were higher than CellEffic cord blood. In addition, CD45+CD71+ cells were lower, whereas CD45+CD34+CD38? cells were higher for the CellEffic cord blood system. Conclusion: Despite the fact that there is a need for well-trained personnel for processing cord blood units with CellEffic cord blood, it may be an attractive alternative to Sepax system for cord blood processing, particularly for cord blood units with low volumes, at banks with low budget where the cord blood turnover rates are relatively low.en_US
dc.identifier.citationMESUTOĞLU P. Y,ÖNCÜL E. B. G,AKIN H. Y,BEKSAÇ M (2022). Manual Versus Automated Volume Reduction of Cord Blood. European Journal of Therapeutics, 28(2), 102 - 108. Doi: 10.54614/eurjther.2021.0029en_US
dc.identifier.doi10.54614/eurjther.2021.0029en_US
dc.identifier.endpage108en_US
dc.identifier.issn2564-7784en_US
dc.identifier.issue2en_US
dc.identifier.startpage102en_US
dc.identifier.trdizinid531216en_US
dc.identifier.urihttp://doi.org/10.54614/eurjther.2021.0029
dc.identifier.urihttps://hdl.handle.net/20.500.12713/3009
dc.identifier.volume28en_US
dc.identifier.wosWOS:000865982100004en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorYurdakul Mesutoğlu, Pınar
dc.language.isoenen_US
dc.publisherTR- Dizinen_US
dc.relation.ispartofEuropean Journal of Therapeuticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHematopoietic Stem Cellsen_US
dc.subjectCord Blooden_US
dc.subjectBlood Bankingen_US
dc.titleManual versus automated volume reduction of cord blooden_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
092be0aa-eee1-4ffc-a593-8acc01cf0686.pdf
Boyut:
1.39 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu
Temel Tıp Bilimleri Bölümü Makale Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu