Differential Cytotoxic Activity of a New Cationic Pd(II) Coordination Compound with N4-Tetradentate Hybrid Ligand in Cancer Cell Lines

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dc.contributor.authorGenel, M.E.
dc.contributor.authorSelvi, S.
dc.contributor.authorYilmaz, I.
dc.contributor.authorAkar, R.O.
dc.contributor.authorYaylim, I.
dc.contributor.authorSengul, A.
dc.contributor.authorUlukaya E.
dc.date.accessioned2024-05-19T14:33:20Z
dc.date.available2024-05-19T14:33:20Z
dc.date.issued2022
dc.departmentİstinye Üniversitesien_US
dc.description.abstractObjective: Successful cancer treatment still requires the discovery of novel compounds that hold promise for chemotherapeutics. The objective of this study was to examine the effectiveness of a newly synthesized cationic palladium(II) coordination compound that functions via several pathways to provide an efficient therapeutic option for various cancer cells. Materials and Methods: A new cationic palladium(II) coordination compound, [Pd(L)]Cl2·H2O, denoted as Complex 1, where the ligand L is the compound 6,6'-bis(NH-benzimidazol-2-yl)-2,2'-bipyridine), was synthesized and characterized by the attenuated total reflectance (ATR)-fourier-transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (1H NMR), electrospray ionization mass spectrometry (ESI-MS), and carbon-hydrogen-nitrogen (CHN) analyses. The density functional theory (DFT) calculations show the coordination sphere around the metal center in Complex 1 to be made up of tertiary N atoms of the pyridine (py) and benzimidazole (bim) rings completing the square-planar geometry with significant distortion. The anti-growth/cytotoxic activity of the complex was determined using the sulforhodamine B (SRB) and adenosine triphosphate (ATP) viability assays for 24 and 48 h in vitro. The study evaluates the determinations for annexin V-propidium iodide (PI) positivity, mitochondrial membrane potential loss, Bcl-2 protein inactivation, and deoxyribonucleic acid (DNA) damage to investigate the cell death mode and its partial mechanism. Results: Complex 1 caused cytotoxicity in a dose-dependent manner in all the cell lines used, with IC50 values ranging from 2.6-8.8 ?M for 48 h. Among the cancer models, colon and breast cancer cell lines underwent cell death by well-described apoptosis through the intrinsic pathway involving the mitochondria. However, the other cell lines did not show such a cell death modality. This implies that differential cell death modes operate based on the cancer type. Conclusion: For the treatment of breast and colon cancers, the complex 1 appears to be a unique, promising complex. Therefore, complex 1 deserves further attention for proof of concept in animal models. © 2022, Istanbul University Press. All rights reserved.en_US
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK: 104T060, TBAG-2450en_US
dc.description.sponsorshipFinancial Disclosure: This work was partly supported by the Turkish Scientific and Technical Research Council (TÜBİTAK) (grant number TBAG-2450(104T060)).en_US
dc.identifier.doi10.26650/experimed.1188586
dc.identifier.endpage201en_US
dc.identifier.issn2667-5846
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85173965270en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage188en_US
dc.identifier.trdizinid1167561en_US
dc.identifier.urihttps://doi.org/10.26650/experimed.1188586
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1167561
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4192
dc.identifier.volume12en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherIstanbul University Pressen_US
dc.relation.ispartofExperimeden_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectAnticancer Effecten_US
dc.subjectApoptosisen_US
dc.subjectBenzimidazoleen_US
dc.subjectBipyridineen_US
dc.subjectN4-Donor Liganden_US
dc.subjectPalladium Complexesen_US
dc.titleDifferential Cytotoxic Activity of a New Cationic Pd(II) Coordination Compound with N4-Tetradentate Hybrid Ligand in Cancer Cell Linesen_US
dc.typeArticleen_US

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