Synthesis and Anticancerogenic Effect of New Generation Ruthenium-Based Nanoparticle from Homalothecium sericeum with Eco-Friendly Method

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dc.authoridAltuner, Ergin Murat/0000-0001-5351-8071
dc.authoriderdem kuruca, serap/0000-0001-7878-9994
dc.authoridkariper, ishak afsin/0000-0001-9127-301X
dc.authoridUlukaya, Engin/0000-0003-4875-5472
dc.authoridOzerkan, Dilsad/0000-0002-0556-3879
dc.authorwosidAltuner, Ergin Murat/AAD-5489-2019
dc.contributor.authorSamir, Nourhan
dc.contributor.authorOzerkan, Dilsad
dc.contributor.authorDanisman-Kalindemirtas, Ferdane
dc.contributor.authorKariper, Ishak Afsin
dc.contributor.authorBulut, Huri
dc.contributor.authorKuruca, Duerdane Serap
dc.contributor.authorAltuner, Ergin Murat
dc.date.accessioned2024-05-19T14:46:33Z
dc.date.available2024-05-19T14:46:33Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractBackgroundGreen synthesis is a simple, inexpensive, and highly efficient method for the preparation of nanoparticles. In this study, ethanol extracts of Homalothecium sericeum (HOM) moss were used as reducing agents for the synthesis of biocompatible ruthenium nanoparticles (RuNPs). The ruthenium-based green synthesis method has not been used in any other work in the literature. UV-visible spectrophotometer (UV-Vis), Zetasizer, FTIR, and EDX-SEM were used to characterize the RuNPs synthesized by the green synthesis method, and their efficacy on cell viability was tested on HCT116 human colon cancer cells.MethodsUV spectroscopic measurements were used to study the release of HOM-RuNPs. Apoptosis was assessed by measuring protein expression of p53, Bax, and Bcl-2 by Western blotting. The presence of apoptosis was confirmed by double staining with Hoechst dye/propidium iodide under a fluorescence microscope. HOM-RuNPs were also tested for BCRP/ABCG2 expression to check for drug resistance.ResultsHOM-RuNPs with a size of 70-80 nm were found to be most effective at a dosage of 5.71 mu g/ml and induced cell death by increasing the ratio of Bax/Bcl-2 and p53 expression. It was also shown to reduce multidrug resistance protein (ABCG2), suggesting that it may be useful against multidrug resistance.ConclusionRuthenium-based nanoparticles synthesized by a green synthesis technique may be a candidate for anticancer drugs in the pharmaceutical industry and deserve further attention for proof-of-concept studies.en_US
dc.description.sponsorshipIstinye University [2020/B4]en_US
dc.description.sponsorshipThis study is supported by the project numbered 2020/B4, Istinye University.en_US
dc.identifier.doi10.1007/s12247-023-09725-6
dc.identifier.endpage767en_US
dc.identifier.issn1872-5120
dc.identifier.issn1939-8042
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85160802315en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage756en_US
dc.identifier.urihttps://doi.org10.1007/s12247-023-09725-6
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5548
dc.identifier.volume18en_US
dc.identifier.wosWOS:000999249800001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Pharmaceutical Innovationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectRuthenium Nanoparticlesen_US
dc.subjectGreen Synthesisen_US
dc.subjectApoptosisen_US
dc.subjectHomalothecium Sericeumen_US
dc.titleSynthesis and Anticancerogenic Effect of New Generation Ruthenium-Based Nanoparticle from Homalothecium sericeum with Eco-Friendly Methoden_US
dc.typeArticleen_US

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