Biohybrids for Combined Therapies of Skin Wounds: Agglomerates of Mesenchymal Stem Cells with Gelatin Hydrogel Beads Delivering Phages and Basic Fibroblast Growth Factor

Basit Öğe Kaydı
dc.authorscopusidErdal Karaöz / 7003448087
dc.authorwosidErdal Karaöz / AAO-1470-2020
dc.contributor.authorMoghtader, Farzaneh
dc.contributor.authorTabata, Yasuhiko
dc.contributor.authorKaraöz, Erdal
dc.date.accessioned2025-04-18T10:17:46Z
dc.date.available2025-04-18T10:17:46Z
dc.date.issued2024
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü
dc.description.abstractThere is great interest in developing effective therapies for the treatment of skin wounds accompanied by deep tissue losses and severe infections. We have attempted to prepare biohybrids formed of agglomerates of mesenchymal stem cells (MSCs) with gelatin hydrogel beads (GEL beads) delivering bacteriophages (phages) as antibacterial agents and/or basic fibroblast growth factor (bFGF) for faster and better healing, providing combined therapies for these types of skin wounds. The gelatin beads were produced through a two-step process using basic and/or acidic gelatins with different isoelectric points. Escherichia coli (E. coli) and its specific T4 phages were propagated. Phages and/or bFGF were loaded within the GELs and their release rates and modes were obtained. The phage release from the basic GEL beads was quite fast; in contrast, the bFGF release from the acidic GEL beads was sustained, as anticipated. MSCs were isolated from mouse adipose tissues and 2D-cultured. Agglomerates of these MSCs with GEL beads were formed and maturated in 3D cultures, and their time-dependent changes were followed. In these 3D culture experiments, it was observed that the agglomerates with GEL beads were very healthy and the MSCs formed tissue-like structures in 7 days, while the MSC agglomerates were not healthy and shrunk considerably as a result of cell death.
dc.description.sponsorshipKyoto University
dc.identifier.citationMoghtader, F., Tabata, Y., & Karaöz, E. (2024). Biohybrids for Combined Therapies of Skin Wounds: Agglomerates of Mesenchymal Stem Cells with Gelatin Hydrogel Beads Delivering Phages and Basic Fibroblast Growth Factor. Gels, 10(8), 493.
dc.identifier.doi10.3390/gels10080493
dc.identifier.issn23102861
dc.identifier.issue8
dc.identifier.scopus2-s2.0-85202610911
dc.identifier.scopusqualityQ2
dc.identifier.urihttp://dx.doi.org/10.3390/gels10080493
dc.identifier.urihttps://hdl.handle.net/20.500.12713/7013
dc.identifier.volume10
dc.identifier.wosWOS:001305773300001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakScopus
dc.indekslendigikaynakWeb of Science
dc.institutionauthorKaraöz, Erdal
dc.institutionauthoridErdal Karaöz / 0000-0002-9992-833X
dc.language.isoen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.relation.ispartofGels
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectİnfected Skin Wounds
dc.subjectBiohybrids
dc.subjectMesenchymal Stem Cells
dc.subjectGelatin Hydrogel Beads
dc.subjectBacteriophages (Phages)
dc.subjectBasic Fibroblast Growth Factor
dc.titleBiohybrids for Combined Therapies of Skin Wounds: Agglomerates of Mesenchymal Stem Cells with Gelatin Hydrogel Beads Delivering Phages and Basic Fibroblast Growth Factor
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
gels-10-00493.pdf
Boyut:
2.24 MB
Biçim:
Adobe Portable Document Format
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.17 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Dahili Tıp Bilimleri Bölümü Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu