Molecular characterisation and potential pathogenicity analysis of Acanthamoeba isolated from recreational lakes in Peninsular Malaysia

Basit Öğe Kaydı
dc.contributor.authorHalim, R.A.
dc.contributor.authorHussain, R.H.M.
dc.contributor.authorAazmi, S.
dc.contributor.authorHalim, H.
dc.contributor.authorKhan, N.A.
dc.contributor.authorSiddiqui, R.
dc.contributor.authorAnuar T.S.
dc.date.accessioned2024-05-19T14:33:15Z
dc.date.available2024-05-19T14:33:15Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractThe present study aims to identify the Acanthamoeba genotypes and their pathogenic potential in three recreational lakes in Malaysia. Thirty water samples were collected by purposive sampling between June and July 2022. Physical parameters of water quality were measured in situ while chemical and microbiological analyses were performed in the laboratory. The samples were vacuum filtered through nitrate filter, cultured onto non-nutrient agar and observed microscopically for amoebic growth. DNAs from positive samples were extracted and made to react with polymerase chain reaction using specific primers. Physiological tolerance tests were performed for all Acanthamoeba-positive samples. The presence of Acanthamoeba was found in 26 of 30 water samples by PCR. The highest rate in lake waters contaminated with amoeba was in Biru Lake (100%), followed by Titiwangsa Lake (80%) and Shah Alam Lake (80%). ORP, water temperature, pH and DO were found to be significantly correlated with the presence of Acanthamoeba. The most common genotype was T4. Temperature- and osmo-tolerance tests showed that 8 (30.8%) of the genotypes T4, T9 and T11 were highly pathogenic. The presence of genotype T4 in habitats related to human activities supports the relevance of this amoeba as a potential public health concern. © 2023 The Authorsen_US
dc.description.sponsorship600-RMC/GIP 5/3[088/2022]; Ministry of Higher Education, Malaysia, MOHE; Universiti Teknologi MARA, UiTMen_US
dc.description.sponsorshipThis study was financially supported by the Fundamental Research Grant Scheme (FRGS/1/2022/SKK0/UITM/02/13), Ministry of Higher Education, Malaysia and Geran Inisiatif Penyeliaan (600-RMC/GIP 5/3[088/2022]), Universiti Teknologi MARA, Malaysia.en_US
dc.identifier.doi10.2166/wh.2023.186
dc.identifier.endpage1356en_US
dc.identifier.issn1477-8920
dc.identifier.issue9en_US
dc.identifier.pmid37756200en_US
dc.identifier.scopus2-s2.0-85174307988en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1342en_US
dc.identifier.urihttps://doi.org/10.2166/wh.2023.186
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4157
dc.identifier.volume21en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherIWA Publishingen_US
dc.relation.ispartofJournal of Water and Healthen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectAcanthamoebaen_US
dc.subjectGenotypeen_US
dc.subjectLakeen_US
dc.subjectMalaysiaen_US
dc.subjectPhysiological Toleranceen_US
dc.titleMolecular characterisation and potential pathogenicity analysis of Acanthamoeba isolated from recreational lakes in Peninsular Malaysiaen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu