Assessment of external calibration, internal standard calibration and quadruple isotope dilution strategies for the determination of acrylamide in wastewater samples after LC-MS/MS quantification

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dc.authoridERARPAT BODUR, Sezin/0000-0002-9879-6662
dc.authoridBAKIRDERE, Sezgin/0000-0001-9746-3682
dc.authoridBodur, Süleyman/0000-0002-2994-9047
dc.authorwosidERARPAT BODUR, Sezin/AAZ-6733-2020
dc.authorwosidBAKIRDERE, Sezgin/ABU-6359-2022
dc.authorwosidBodur, Süleyman/AHB-4581-2022
dc.contributor.authorYazici, Elif
dc.contributor.authorBodur, Suleyman
dc.contributor.authorErarpat, Sezin
dc.contributor.authorArslan, Ulas Emre
dc.contributor.authorBakirdere, Sezgin
dc.date.accessioned2024-05-19T14:38:48Z
dc.date.available2024-05-19T14:38:48Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractAcrylamide is a possible neurotoxicant and carcinogen compound and its common usage in various industrial processes causes acrylamide contamination in environmental bodies. For this purpose, there is an increasing demand to develop an accurate and selective analytical method for the determination of acrylamide. In the scope of this study, external calibration (EC), internal standard calibration (ISC) and quadruple isotope dilution (ID4) strategies were compared to each other in terms of accuracy and precision. After the evaluation of these methods, liquid chromatography - quadruple isotope dilution - tandem mass spectrometry (LC-ID4-MS/MS) as a highly accurate and precise analytical approach was proposed to quantify acrylamide in wastewater samples. Several parameters for LC system such as sample injection volume, mobile phase type/composition and mobile phase flow rate were elaborately optimized to enhance peak shape and signal to noise ratio (S/N) belonging to the analyte. Under the optimum chromatographic and mass determination conditions, system analytical perfor-mance study was performed to determine dynamic range and limit of detection/quantitation (LOD and LOQ). LOD and LOQ values of the proposed LC-MS/MS method for the analyte were calculated as 1.18 and 3.93 mu g/kg; respectively. After the system analytical performance study, the spiking experiments in wastewater samples were carried out for the evaluation of accuracy and applicability of LC-MS/MS method. For wastewater samples, percent recovery results with their percent relative standard deviations (%RSD) obtained from EC, ISC and ID4 strategies were 115.8-177.8% (+/- 1.2-11.4%), 94.5-117.7% (0.3-4.7%) and 99.6-100.9% (0.4-1.7%); respec-tively. These results prove the superiority of ID4 strategy with regards to accuracy and precision of the results.en_US
dc.identifier.doi10.1016/j.microc.2023.108741
dc.identifier.issn0026-265X
dc.identifier.issn1095-9149
dc.identifier.scopus2-s2.0-85152288862en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org10.1016/j.microc.2023.108741
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4612
dc.identifier.volume190en_US
dc.identifier.wosWOS:000988780900001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofMicrochemical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectAcrylamideen_US
dc.subjectQuadruple Isotope Dilutionen_US
dc.subjectWastewateren_US
dc.subjectLc-Msen_US
dc.subjectMsen_US
dc.titleAssessment of external calibration, internal standard calibration and quadruple isotope dilution strategies for the determination of acrylamide in wastewater samples after LC-MS/MS quantificationen_US
dc.typeArticleen_US

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