Usnea filipendula induces apoptosis in human colon cancer cell lines

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dc.authoridEngin Ulukaya / 0000-0003-4875-5472
dc.authoridMerve Erkısa Genel / 0000-0002-3127-742X
dc.authorscopusidEngin Ulukaya / 6602927353
dc.authorscopusidMerve Erkısa Genel / 57126208900
dc.authorwosidEngin Ulukaya / K-5792-2018
dc.authorwosidMerve Erkısa Genel / AAM-1001-2020
dc.contributor.authorDisoma, C.
dc.contributor.authorErkısa Genel, Merve
dc.contributor.authorOran, S.
dc.contributor.authorAlioğlu, I.
dc.contributor.authorUlukaya, Engin
dc.contributor.authorArı, F.
dc.date.accessioned2020-08-30T20:07:15Z
dc.date.available2020-08-30T20:07:15Z
dc.date.issued2018
dc.departmentİstinye Üniversitesi, Rektörlüken_US
dc.description.abstractLichens are complex organisms living in a symbiotic relationship with fungi and algae have recently received special interest in cancer research. The cytotoxic activities of Usnea filipendula Stirt. lichen extract was investigated on colon cancer cell lines, HCT-15 and HT-29. Sulphorhodamine B and ATP cell viability tests were used to monitor cytotoxic activity. The mode of cell death (apoptosis/necrosis) was determined using caspase-cleaved cytokeratin 18 (M30), caspase-3/7 activity and fluorescence staining techniques that included, Annexin-V, Hoechst 33342 and propidium iodide. Usnea filipendula showed dose and time-dependent antiproliferative effect in HCT-15 and HT-29 cells. The IC50 values in HCT-15 and HT-29 cells were 17.92 and 41.87 mu g/ml, respectively. The extract induced apoptosis in both cell lines especially in HCT-15 cells in which caspase-3/7 activity was increased. Usnea filipendula was cytotoxic to colon cancer HCT-15 and HT-29 cell lines by inducing early or late apoptosis as evidenced by translocation of phosphatidylserine, pyknotic nuclei and nuclear condensation. Further studies would help to understand the full potential of Usnea filipendula as a novel anticancer therapy.en_US
dc.description.sponsorshipUludag UniversityUludag University [BUAP(F)-2014/3]en_US
dc.description.sponsorshipAuthors gratefully the Research Fund of Uludag University for the project that is numbered BUAP(F)-2014/3. The authors would also like to thank Dr Serap Celikler (Uludag University, Bursa, Turkey) for contributing to the extraction and lyophilization of the lichens.en_US
dc.identifier.citationDisoma, C., Erkisa, M., Oran, S., Alioglu, I., Ulukaya, E., & Ari, F. (2018). Usnea filipendula Induces Apoptosis in Human Colon Cancer Cell Lines. INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES, 80(6), 1078–1085. https://doi.org/10.4172/pharmaceutical-sciences.1000458en_US
dc.identifier.doi10.4172/pharmaceutical-sciences.1000458en_US
dc.identifier.endpage1085en_US
dc.identifier.issn0250-474Xen_US
dc.identifier.issn1998-3743en_US
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85110696311en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage1078en_US
dc.identifier.urihttps://doi.org/10.4172/pharmaceutical-sciences.1000458
dc.identifier.urihttps://hdl.handle.net/20.500.12713/728
dc.identifier.volume80en_US
dc.identifier.wosWOS:000455184700012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorErkısa Genel, Merveen_US
dc.institutionauthorUlukaya, Enginen_US
dc.language.isoenen_US
dc.publisherWolters Kluwer Medknow Publicationsen_US
dc.relation.ispartofIndian Journal of Pharmaceutical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectUsnea Filipendulaen_US
dc.subjectApoptosisen_US
dc.subjectColon Canceren_US
dc.subjectCancer Cell Deathen_US
dc.titleUsnea filipendula induces apoptosis in human colon cancer cell linesen_US
dc.typeArticleen_US

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