COMPOSITION ANALYSIS OF E-LIQUIDS AND THEIR EFFECTS ON HEALTHY LIVER AND PHARYNGEAL CARCINOMA CELL LINES

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dc.authoridYararbas, Gorkem/0000-0002-2993-5577
dc.authoridGeyik, Caner/0000-0002-8382-2186
dc.authorwosidYararbas, Gorkem/HFZ-7629-2022
dc.authorwosidGeyik, Caner/I-8384-2014
dc.contributor.authorGeyik, Caner
dc.contributor.authorGumus, Zinar Pinar
dc.contributor.authorYararbas, Gorkem
dc.date.accessioned2024-05-19T14:42:57Z
dc.date.available2024-05-19T14:42:57Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractElectronic cigarettes have become popular worldwide in recent years although their effects on human health are still not properly known. The lack of regulations brings a problem of inconsistency between ingredients and the product label. We aimed to analyse the contents of widely used e-liquids and their effects on two different cell lines. Eleven e-liquid samples were selected according to their availability and popularity. Nicotine, propylene glycol (PG), glycerine (GLY), and volatile compounds in e-liquids were analysed by High-Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC). 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay was used to determine the effects of e-liquids on transformed human normal liver epithelial cell line (THLE-2) and human pharyngeal carcinoma cell line (Detroit 562). Nicotine amounts were found to be consistent with product labels. and GLY were not only different between brands but also for products within the same brand. THLE-2 cell viability was inversely correlated with e-liquid concentration. However, decreases in cell viability were not correlated with nicotine amount. Interestingly, effects of several samples on Detroit 562 cells were triphasic; decrease in viability at lower doses, cell survival in mid-concentrations and loss of viability in highest doses. The analytical composition of e-liquids differs greatly among products which corresponds to different cellular effects. Viability of cancer cells does not change in a dose-dependent manner, which suggest that cellular differences may play role in the outcome of these products.en_US
dc.description.sponsorshipScientific and Technological Research Council of Tuerkiye (TUBITAK) [114Z687]; Ege University Science and Technology Centre [15-BIL-033]en_US
dc.description.sponsorshipThe study was supported by The Scientific and Technological Research Council of Tuerkiye (TUBITAK) with project number 114Z687 and Ege University Science and Technology Centre with the project number 15-BIL-033.en_US
dc.identifier.doi10.23902/trkjnat.1135237
dc.identifier.endpage29en_US
dc.identifier.issn2528-9691
dc.identifier.issue1en_US
dc.identifier.startpage21en_US
dc.identifier.trdizinid1164024en_US
dc.identifier.urihttps://doi.org10.23902/trkjnat.1135237
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1164024
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5304
dc.identifier.volume24en_US
dc.identifier.wosWOS:000981245300004en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherTrakya Univ Balkan Yerlesesi Enstituler Binasien_US
dc.relation.ispartofTrakya University Journal of Natural Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectKey Electronic Cigarettes Electronic Liquids Chromatographic Analysis Cell Viabilityen_US
dc.titleCOMPOSITION ANALYSIS OF E-LIQUIDS AND THEIR EFFECTS ON HEALTHY LIVER AND PHARYNGEAL CARCINOMA CELL LINESen_US
dc.typeArticleen_US

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