Polymeric approach to adjuvant system in antibody production against leishmaniasis based on hybridoma technology

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dc.authoridAslı Pınar Zorba Yıldız / 0000-0001-6854-9890en_US
dc.authorscopusidAslı Pınar Zorba Yıldız / 57626992600en_US
dc.authorwosidAslı Pınar Zorba Yıldız / L-6429-2018en_US
dc.contributor.authorYıldız, Aslı Pınar Zorba
dc.contributor.authorKöken, Gülnaz Yıldırım
dc.contributor.authorAbamor, Emrah Şefik
dc.contributor.authorBağırova, Melahat
dc.contributor.authorTosyalı, Özlem Ayşe
dc.contributor.authorKocagöz, Tanil
dc.contributor.authorAllahverdiyev, Adil
dc.date.accessioned2023-02-03T06:53:41Z
dc.date.available2023-02-03T06:53:41Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Laboratuvar Teknikleri Programıen_US
dc.description.abstractBackground: Leishmaniasis is a zoonotic disease, which is one of the serious public health problems in the world. Nowadays, antibody production using hybridoma technology may be a correct approach in terms of sensitivity in the diagnosis of diseases such as leishmaniasis. The aim of this study was investigation of the effectiveness of different adjuvants on polyclonal antibody production against L. tropica based on hybridoma technique.Methods: Accordingly, Freund's adjuvant (1956, M. tuberculosis), as a classic adjuvant in studies, was used comparatively with the non-toxic polymeric based Polyoxidonium adjuvant. All animal immunization procedures were conducted at Bezm-i Alem University Experimental Animal Research Center. The adjuvant response was tested both in the serum sample and in the antibodies produced by the hybridomas. The antibody titers were determined with ELISA.Results: Freund's and Polyoxidonium (PO) group blood titer's increased approximately 5.5 fold compared to control after the 6(th) and 8(th) immunization. Hybridomas produced from mice immunized with PO adjuvant induced only antigen-specific antibody response and did not develop an immune response against the adjuvant.Conclusion: Adjuvant selection is very important in terms of the specificity of antibody responses of cells produced in hybridoma technology. Therefore, PO is recommended as a new adjuvant system in this study.en_US
dc.identifier.citationYildiz, A. P. Z., Koken, G. Y., Abamor, E. S., Bagirova, M., Tosyali, O. A., Kocagoz, T., & Allahverdiyev, A. (2022). Polymeric Approach to Adjuvant System in Antibody Production against Leishmaniasis Based on Hybridoma Technology. Iranian Journal of Parasitology, 17(4), 506-516.en_US
dc.identifier.endpage516en_US
dc.identifier.issn1735-7020en_US
dc.identifier.issn2008-238Xen_US
dc.identifier.issue4en_US
dc.identifier.startpage506en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12713/3859
dc.identifier.volume17en_US
dc.identifier.wosWOS:000900875600007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.institutionauthorZorba Yıldız, Aslı Pınar
dc.language.isoenen_US
dc.publisherTehran University of Medical Sciencesen_US
dc.relation.ispartofIRANIAN JOURNAL OF PARASITOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLeishmaniasisen_US
dc.subjectAntibodyen_US
dc.subjectHybridoma Technologyen_US
dc.subjectAdjuvanten_US
dc.subjectVaccineen_US
dc.titlePolymeric approach to adjuvant system in antibody production against leishmaniasis based on hybridoma technologyen_US
dc.typeArticleen_US

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