Regulation of MMP 2 and MMP 9 expressions modulated by AP-1 (c-jun) in wound healing: improving role of Lucilia sericata in diabetic rats

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dc.authoridFatma Kübra Tombultürk / 0000-0002-4358-2309en_US
dc.authorscopusidFatma Kübra Tombultürk / 56748052500
dc.authorwosidFatma Kübra Tombultürk / L-3969-2018
dc.contributor.authorTombultürk, Fatma Kübra
dc.contributor.authorSoydaş, Tuğba
dc.contributor.authorSaraç, Elif Yaprak
dc.contributor.authorTunçdemir, Matem
dc.contributor.authorCoşkunpınar, Ender
dc.contributor.authorPolat, Erdal
dc.contributor.authorSirekbasan, Serhat
dc.contributor.authorKanigur-Sultuybek, Gönül
dc.date.accessioned2020-08-30T20:07:00Z
dc.date.available2020-08-30T20:07:00Z
dc.date.issued2019
dc.departmentİstinye Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Laboratuvar Teknikleri Bölümüen_US
dc.descriptionSirekbasan, Serhat/0000-0001-7967-3539; Tuncdemir, Matem/0000-0002-5300-4449; Tombulturk, Fatma Kubra/0000-0002-4358-2309en_US
dc.description.abstractAimsLucilia sericata larvae have been successfully used on healing of wounds in the diabetics. However, the involvement of the extraction/secretion (ES) products of larvae in the treatment of diabetic wounds is still unknown. Activator protein-1 (AP-1) transcription, composed of c-jun and c-Fos proteins, has been shown to be the principal regulator of multiple MMP transcriptions under a variety of conditions, also in diabetic wounds. Specifically, MMP-2 and MMP-9's transcriptions are known to be modulated by AP-1. c-jun has been demonstrated to be a repressor of p53 in immortalized fibroblasts. The aim of the present study is to investigate the effects of L. sericata ES on the expression of AP-1 (c-jun), p53, MMP-2, and MMP-9 in wound biopsies dissected from streptozotocin induced diabetic rats.MethodsThe expression levels of MMP-2, MMP-9, c-jun and p53 in dermal tissues were determined at days 0, 3, 7 and 14 after wounding, using immunohistochemical analysis and quantitative real-time PCR.ResultsThe treatment with ES significantly decreased through inflammation-based induction of MMP-2 and MMP-9 expression levels in the wounds of diabetic groups, compared to control groups at the third day of wound healing. At the 14th day, there were dramatic decreases in expression of c-jun, MMP-9, and p53 in ES-treated groups, compared to the diabetic group (P<0.001, P<0.05 and P<0.01, respectively).ConclusionES products of L. sericata may enhance the process of wound healing in phases of inflammation, proliferation, and re-epithelization, essentially via regulating c-jun expression and modulating MMP-2 and MMP-9 expressions.en_US
dc.description.sponsorshipScientific Research Project Coordination Unit of Istanbul UniversityIstanbul University [35048]en_US
dc.description.sponsorshipThe concept and design of research belong to FKT and GSK. Animal experiments were carried out by FKT and MT, ES preparation by FKT, EP, and SS, RT-PCR was conducted by FKT and EC. TS and EYS carried out the immunohistochemical analysis. FKT, TS, and EYS performed data analysis, interpretation and writing the article, supervised by GSK. This work was supported by the Scientific Research Project Coordination Unit of Istanbul University (Project number: 35048).en_US
dc.identifier.citationTombulturk, F. K., Soydas, T., Sarac, E. Y., Tuncdemir, M., Coskunpinar, E., Polat, E., … Kanigur-Sultuybek, G. (2019). Regulation of MMP 2 and MMP 9 expressions modulated by AP-1 (c-jun) in wound healing: improving role of Lucilia sericata in diabetic rats. ACTA DIABETOLOGICA, 56(2), 177–186. https://doi.org/10.1007/s00592-018-1237-5en_US
dc.identifier.doi10.1007/s00592-018-1237-5en_US
dc.identifier.endpage186en_US
dc.identifier.issn0940-5429en_US
dc.identifier.issn1432-5233en_US
dc.identifier.issue2en_US
dc.identifier.pmid30302545en_US
dc.identifier.scopus2-s2.0-85054789427en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage177en_US
dc.identifier.urihttps://doi.org/10.1007/s00592-018-1237-5
dc.identifier.urihttps://hdl.handle.net/20.500.12713/672
dc.identifier.volume56en_US
dc.identifier.wosWOS:000458279100006en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorTombultürk, Fatma Kübraen_US
dc.language.isoenen_US
dc.publisherSpringer-Verlag Italia Srlen_US
dc.relation.ispartofActa Diabetologicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectStz Diabetesen_US
dc.subjectMaggot Debridement Therapyen_US
dc.subjectLucilia Sericataen_US
dc.subjectMmpen_US
dc.subjectP53en_US
dc.subjectAp-1en_US
dc.titleRegulation of MMP 2 and MMP 9 expressions modulated by AP-1 (c-jun) in wound healing: improving role of Lucilia sericata in diabetic ratsen_US
dc.typeArticleen_US

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