SDF-1 modulates periodontal ligament-Mesenchymal Stem Cells (pdl-MSCs)
Yükleniyor...
Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
WILEY
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background/objectives: In this in vitro study, the effects of Stromal cell-derived factor-1 (SDF-1) was evaluated on the periodontal ligament-Mesenchymal Stem Cells (pdl-MSCs) functions. Material and methods: Real-time cell analyzer-single plate (RTCA-SP) was employed for proliferation, and RTCA-dual purpose (DP) was utilized for pdl-MSCs migration potential treated with different SDF-1 concentrations (0, 0.1, 1, 10, 100, 200, and 400 ng/ml). Based on the dose-response findings, 10 ng/ml SDF-1 was used for further mRNA experiments. RNAs isolated at 6 and 24 h were checked using quantitative RT-PCR for mineralized tissue-associated genes including type I collagen (COL I), osteocalcin (OCN), osteopontin (OPN), and runt-related transcription factor 2 (Runx2). cRNA was synthesized for 6 h, and whole-genome array analysis was performed for over 47.000 probes. Data were subjected to quantile normalization before analysis. Results: Increased proliferation and migration were observed in pdl-MSCs treated with 0.1, 1, and 10 ng/ml SDF-1. Increased COL I was observed at both time points: 6 and 24 h. While there was no significant change for OCN, OPN, and Runx2 at 6 h, SDF-1 up-regulated OCN and OPN, but down-regulated Runx2 mRNA expressions at 24 h. IL-8 and ESM1 genes were differentially expressed over twofold when the pdl-MSCs were exposed to SDF-1 at whole-genome array analysis. IL-8 induction was confirmed with RT-PCR. Conclusion: Findings of this study displayed that SDF-1 modulated pdl-MSCs which were important for periodontal regeneration, inducing migration and proliferation, and regulating extracellular matrix synthesis in favor of the formation of new attachment.
Açıklama
Anahtar Kelimeler
IL-8, MRNA Expression, Periodontal Ligament-Mesenchymal Stem Cells, Real-Time Migration, Real-Time Proliferation, Stromal Cell-Derived Factor-1, Whole Genome Array
Kaynak
J Periodontal Res.
WoS Q Değeri
Q1
Scopus Q Değeri
N/A
Cilt
Sayı
Künye
Hakki, S. S., Bozkurt, B. S., Hakki, E. E., Karaoz, E., Unlu, A., & Kayis, S. A. (2021). SDF-1 modulates periodontal ligament-Mesenchymal Stem Cells (pdl-MSCs). Journal of periodontal research, 10.1111/jre.12876. Advance online publication. https://doi.org/10.1111/jre.12876
