The therapeutic efficacy of 5-ALA based photodynamic therapy and chemotherapy combination in triple negative breast cancer cells

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dc.authorscopusidBeyzanur Erk / 57789532000
dc.authorwosidBeyzanur Erk / CNN-5660-2022
dc.contributor.authorErk, Beyzanur
dc.contributor.authorKamanlı, Ali Furkan
dc.contributor.authorEskiler, Gamze Güney
dc.date.accessioned2025-04-18T07:43:57Z
dc.date.available2025-04-18T07:43:57Z
dc.date.issued2024
dc.departmentİstinye Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümü
dc.description.abstractTriple negative breast cancer (TNBC) is one of the subtypes of breast cancer characterized by a heterogeneous and aggressive nature. Photodynamic therapy (PDT) has drawn significant attention in cancer treatment. However, solubility of photosensitizer, penetration problems into a target tissue and insufficient oxygen concentration limit the effectiveness of PDT. To overcome these limitations and to reduce the side effects of chemotherapy, combination treatment modalities play an essential role in cancer treatment. In this study, we aimed to investigate the combination efficacy of cisplatin-based chemotherapy and 5-Aminolevulinic acid (5-ALA)/PDT in TNBC cells and healthy breast cells in vitro. To determine the effect of the combination effects of cisplatin and 5-ALA/PDT on TNBC cells, two treatment protocols (simultaneous and sequential combination therapy) were evaluated compared with cisplatin and 5-ALA/PDT monotherapy and WST-1, Annexin V assay, acridine orange (AO) and mitochondrial staining were performed. Our findings showed that MDA-MB-231 TNBC cell viability was significantly decreased following simultaneous combination treatment compared to cisplatin and 5-ALA/PDT monotherapy. Additionally, simultaneous combination treatment was more effective than sequential combination treatment. The simultaneous combination treatment of 2.5 mu M cisplatin and 5-ALA/PDT at 6 J/cm2 and 9 J/cm2 induced 46.78% and 53.6% total apoptotic death, respectively in TNBC cells compared with monotherapies (cisplatin (37.88%) and 5-ALA/PDT (6 J/cm2: 31.48% and 9 J/cm2: 37.78%). Additionally, cisplatin and 5-ALA/PDT combination treatment resulted in nuclear fragmentation and mitochondrial damage due to apoptosis. Our results suggest that cisplatin and 5-ALA/PDT simultaneous combination therapy could be a promising new alternative strategy for treating TNBC. However, further studies are required to assess the underlying molecular mechanisms of cisplatin and 5-ALA/PDT combination treatment at the molecular level.
dc.description.sponsorshipScientific and Technological Research Council of Tuerkiye (TUEBITAK) Scientific Research Projects Foundation (BAP) of the Sakarya University of Turkey
dc.identifier.citationErk, B., Kamanli, A. F., & Guney Eskiler, G. (2024). The therapeutic efficacy of 5-ALA based photodynamic therapy and chemotherapy combination in triple negative breast cancer cells. Lasers in Medical Science, 39(1), 191.
dc.identifier.doi10.1007/s10103-024-04141-9
dc.identifier.issn0268-8921
dc.identifier.issn1435-604X
dc.identifier.issue1
dc.identifier.pmid39043901
dc.identifier.scopus2-s2.0-85199272645
dc.identifier.scopusqualityQ1
dc.identifier.urihttp://dx.doi.org/10.1007/s10103-024-04141-9
dc.identifier.urihttps://hdl.handle.net/20.500.12713/6484
dc.identifier.volume39
dc.identifier.wosWOS:001275334800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorErk, Beyzanur
dc.institutionauthoridBeyzanur Erk / 0000-0002-5856-7224
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofLasers in medical science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTriple Negative Breast Cancer
dc.subjectPhotodynamic Therapy
dc.subject5-ALA
dc.subjectCisplatin
dc.subjectApoptosis
dc.titleThe therapeutic efficacy of 5-ALA based photodynamic therapy and chemotherapy combination in triple negative breast cancer cells
dc.typeArticle

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