Lactase can target cellular differentiation of Acanthamoeba castellanii belonging to the T4 genotype

Basit Öğe Kaydı
dc.authoridKhan, Naveed/0000-0001-7667-8553
dc.authoridSiddiqui, Ruqaiyyah/0000-0001-9646-6208
dc.authorwosidKhan, Naveed/AAM-2892-2021
dc.authorwosidKhan, Naveed/KCK-0156-2024
dc.authorwosidSiddiqui, Ruqaiyyah/AIF-2100-2022
dc.contributor.authorSimau, Fathimath Afaaf
dc.contributor.authorAhmed, Usman
dc.contributor.authorKhan, Khalid Mohammed
dc.contributor.authorKhan, Naveed Ahmed
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.contributor.authorAlharbi, Ahmad M.
dc.contributor.authorAlfahemi, Hasan
dc.date.accessioned2024-05-19T14:38:47Z
dc.date.available2024-05-19T14:38:47Z
dc.date.issued2024
dc.departmentİstinye Üniversitesien_US
dc.description.abstractThe free living Acanthamoeba spp. are ubiquitous amoebae associated with potentially blinding disease known as Acanthamoeba keratitis (AK) and a fatal central nervous system infection granulomatous amoebic encephalitis (GAE). With the inherent ability of cellular differentiation, it can phenotypically transform to a dormant cyst form from an active trophozoite form. Acanthamoeba cysts are highly resistant to therapeutic agents as well as contact lens cleaning solutions. One way to tackle drug resistance against Acanthamoeba is by inhibiting the formation of cysts from trophozoites. The biochemical analysis showed that the major component of Acanthamoeba cyst wall is composed of carbohydrate moieties such as galactose and glucose. The disaccharide of galactose and glucose is lactose. In this study, we analyzed the potential of lactase enzyme to target carbohydrate moieties of cyst walls. Amoebicidal assessment showed that lactase was ineffective against trophozoite of A. castellanii but enhanced amoebicidal effects of chlorhexidine. The lactase enzyme did not show any toxicity against normal human keratinocyte cells (HaCaT) at the tested range. Hence, lactase can be used for further assessment for development of potential therapeutic agents in the management of Acanthamoeba infection as well as formulation of effective contact lens disinfectants.en_US
dc.description.sponsorshipAir Force Office of Scientific Researchen_US
dc.description.sponsorshipNo Statement Availableen_US
dc.identifier.doi10.1007/s00436-024-08131-2
dc.identifier.issn0932-0113
dc.identifier.issn1432-1955
dc.identifier.issue2en_US
dc.identifier.pmid38294565en_US
dc.identifier.scopus2-s2.0-85183645425en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org10.1007/s00436-024-08131-2
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4604
dc.identifier.volume123en_US
dc.identifier.wosWOS:001154450900001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofParasitology Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectAcanthamoebaen_US
dc.subjectLactaseen_US
dc.subjectCyst Wallsen_US
dc.titleLactase can target cellular differentiation of Acanthamoeba castellanii belonging to the T4 genotypeen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu