Mesenchymal stem cells differentiate to retinal ganglion-like cells in rat glaucoma model induced by polystyrene microspheres

dc.authoridBOZKURT, SÜHEYLA UYAR/0000-0002-5947-947X
dc.authorwosideraslan, muhsin/E-7686-2016
dc.authorwosidBOZKURT, SÜHEYLA UYAR/JVO-9637-2024
dc.contributor.authorEraslan, Muhsin
dc.contributor.authorCerman, Eren
dc.contributor.authorBozkurt, Suheyla
dc.contributor.authorGenc, Deniz
dc.contributor.authorVirlan, Aysin Tulunay
dc.contributor.authorDemir, Cansu Subasi
dc.contributor.authorAkkoc, Tolga
dc.date.accessioned2024-05-19T14:41:52Z
dc.date.available2024-05-19T14:41:52Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractAim: The study aimed to evaluate the differentiation ability of intravitreally injected rat bone marrow-derived mesenchymal stem cells (rBM-MSCs) to retinal ganglion-like cells in a polystyrene microsphere induced rat glaucoma model.Materials and Methods: The glaucoma rat model was generated via intracameral injection of 7 microliter polystyrene microspheres. Green fluorescence protein-labeled (GFP) rBM-MSCs were transplanted intravitreally at or after induction of ocular hypertension (OHT), depending on the groups. By the end of the fourth week, flat-mount retinal dissection was performed, and labeled against Brn3a, CD90, GFAP, CD11b, Vimentin, and localization of GFP positive rBM-MSCs was used for evaluation through immunofluorescence staining and to count differentiated retinal cells by flow cytometry. From 34 male Wistar albino rats, 56 eyes were investigated.Results: Flow cytometry revealed significantly increased CD90 and Brn3a positive cells in glaucoma induced and with rBM-MSC injected groups compared to control(P = 0.006 and P = 0.003 respectively), sham-operated (P = 0.007 and P < 0.001 respectively), and only rBM-MSCs injected groups (P = 0.002 and P = 0.009 respectively). Immunofluorescence microscopy revealed differentiation of GFP labeled stem cells to various retinal cells, including ganglion-like cells. rBM-MSCs were observable in ganglion cells, inner and outer nuclear retinal layers in rBM-MSCs injected eyes.Conclusion: Intravitreally transplanted rBM-MSCs differentiated into retinal cells, including ganglion-like cells, which successfully created a glaucoma model damaged with polystyrene microspheres. Promisingly, MSCs may have a role in neuro-protection and neuro-regeneration treatment of glaucoma in the future.en_US
dc.identifier.doi10.1016/j.tice.2023.102199
dc.identifier.issn0040-8166
dc.identifier.pmid37633122en_US
dc.identifier.scopus2-s2.0-85168726667en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org10.1016/j.tice.2023.102199
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5171
dc.identifier.volume84en_US
dc.identifier.wosWOS:001070578300001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherChurchill Livingstoneen_US
dc.relation.ispartofTissue & Cellen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectGlaucomaen_US
dc.subjectStem Cellen_US
dc.subjectFlow Cytometryen_US
dc.subjectPolystyrene Microsphereen_US
dc.subjectMicrobeaden_US
dc.subjectNeuro-Protectionen_US
dc.subjectNeuro-Regenerationen_US
dc.subjectRetinal Ganglion Cellen_US
dc.titleMesenchymal stem cells differentiate to retinal ganglion-like cells in rat glaucoma model induced by polystyrene microspheresen_US
dc.typeArticleen_US

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