Mesenchymal stem cells differentiate to retinal ganglion-like cells in rat glaucoma model induced by polystyrene microspheres
dc.authorid | BOZKURT, SÜHEYLA UYAR/0000-0002-5947-947X | |
dc.authorwosid | eraslan, muhsin/E-7686-2016 | |
dc.authorwosid | BOZKURT, SÜHEYLA UYAR/JVO-9637-2024 | |
dc.contributor.author | Eraslan, Muhsin | |
dc.contributor.author | Cerman, Eren | |
dc.contributor.author | Bozkurt, Suheyla | |
dc.contributor.author | Genc, Deniz | |
dc.contributor.author | Virlan, Aysin Tulunay | |
dc.contributor.author | Demir, Cansu Subasi | |
dc.contributor.author | Akkoc, Tolga | |
dc.date.accessioned | 2024-05-19T14:41:52Z | |
dc.date.available | 2024-05-19T14:41:52Z | |
dc.date.issued | 2023 | |
dc.department | İstinye Üniversitesi | en_US |
dc.description.abstract | Aim: The study aimed to evaluate the differentiation ability of intravitreally injected rat bone marrow-derived mesenchymal stem cells (rBM-MSCs) to retinal ganglion-like cells in a polystyrene microsphere induced rat glaucoma model.Materials and Methods: The glaucoma rat model was generated via intracameral injection of 7 microliter polystyrene microspheres. Green fluorescence protein-labeled (GFP) rBM-MSCs were transplanted intravitreally at or after induction of ocular hypertension (OHT), depending on the groups. By the end of the fourth week, flat-mount retinal dissection was performed, and labeled against Brn3a, CD90, GFAP, CD11b, Vimentin, and localization of GFP positive rBM-MSCs was used for evaluation through immunofluorescence staining and to count differentiated retinal cells by flow cytometry. From 34 male Wistar albino rats, 56 eyes were investigated.Results: Flow cytometry revealed significantly increased CD90 and Brn3a positive cells in glaucoma induced and with rBM-MSC injected groups compared to control(P = 0.006 and P = 0.003 respectively), sham-operated (P = 0.007 and P < 0.001 respectively), and only rBM-MSCs injected groups (P = 0.002 and P = 0.009 respectively). Immunofluorescence microscopy revealed differentiation of GFP labeled stem cells to various retinal cells, including ganglion-like cells. rBM-MSCs were observable in ganglion cells, inner and outer nuclear retinal layers in rBM-MSCs injected eyes.Conclusion: Intravitreally transplanted rBM-MSCs differentiated into retinal cells, including ganglion-like cells, which successfully created a glaucoma model damaged with polystyrene microspheres. Promisingly, MSCs may have a role in neuro-protection and neuro-regeneration treatment of glaucoma in the future. | en_US |
dc.identifier.doi | 10.1016/j.tice.2023.102199 | |
dc.identifier.issn | 0040-8166 | |
dc.identifier.pmid | 37633122 | en_US |
dc.identifier.scopus | 2-s2.0-85168726667 | en_US |
dc.identifier.scopusquality | Q2 | en_US |
dc.identifier.uri | https://doi.org10.1016/j.tice.2023.102199 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12713/5171 | |
dc.identifier.volume | 84 | en_US |
dc.identifier.wos | WOS:001070578300001 | en_US |
dc.identifier.wosquality | N/A | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.language.iso | en | en_US |
dc.publisher | Churchill Livingstone | en_US |
dc.relation.ispartof | Tissue & Cell | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.snmz | 20240519_ka | en_US |
dc.subject | Glaucoma | en_US |
dc.subject | Stem Cell | en_US |
dc.subject | Flow Cytometry | en_US |
dc.subject | Polystyrene Microsphere | en_US |
dc.subject | Microbead | en_US |
dc.subject | Neuro-Protection | en_US |
dc.subject | Neuro-Regeneration | en_US |
dc.subject | Retinal Ganglion Cell | en_US |
dc.title | Mesenchymal stem cells differentiate to retinal ganglion-like cells in rat glaucoma model induced by polystyrene microspheres | en_US |
dc.type | Article | en_US |