Cross referencing 2D-LC determination of intact gliptins in urine

Basit Öğe Kaydı
dc.authoridAhmet Cenk Andaç / 0000-0002-4545-3500en_US
dc.authorscopusidAhmet Cenk Andaç / 20336962400
dc.authorwosidAhmet Cenk Andaç / AAW-5539-2020
dc.contributor.authorMohamad, Amal M.
dc.contributor.authorAndaç, Ahmet Cenk
dc.contributor.authorAndaç, Sena Çağlar
dc.date.accessioned2020-09-22T06:25:48Z
dc.date.available2020-09-22T06:25:48Z
dc.date.issued2020en_US
dc.departmentİstinye Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümüen_US
dc.description.abstractDipeptidyl peptidase-4 inhibitors, so-called gliptins, constitute a fairly novel class of oral hypoglycemic agents. The development and validation of an automated online SPE-LC-UV method to determine intact sitagliptin, saxagliptin, vildagliptin and metformin simultaneously in human urine samples were performed. For the two-dimensional chromatographic separation, a Gemini C18 (250.0 × 4.6 mm i.d., 110 A0, 5.0 ?) analytical column and a gradient elution with 10.0 mM o-phosphoric acid and methanol and for the online SPE analysis of urine samples, a LiChrospher® ADS SPE-column (20.0 mm × 2.0 mm i.d., 25.0 ?m) were used through the study. The fractionation, transfer, elution and separation of the spiked urine samples were achieved in just 9.57 min runtime with 12.0 mL of solvent consumption which was green and economical compared to other sample preparation methods. The calibration curves were determined to be linear in a wide range of 0.10-100.00 ?g/mL with satisfactory regression coefficients. Method developed for two-dimensional determination of gliptins would be useful as a reference in therapeutic drug monitoring and screening for forensic medical cases which involve the abuse, unintentional or misuse of multiple gliptins in terms of its practical use, easy detection and reliable results.en_US
dc.identifier.citationMohamad, A. M., Andac, C. A., & Andac, S. C. (2020). Cross Referencing 2D-LC Determination of Intact Gliptins in Urine. Journal of chromatographic science, bmaa059. Advance online publication. https://doi.org/10.1093/chromsci/bmaa059en_US
dc.identifier.doi10.1093/chromsci/bmaa059en_US
dc.identifier.pmid32875329en_US
dc.identifier.scopus2-s2.0-85094684377en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.1093/chromsci/bmaa059
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1058
dc.identifier.wosWOS:000593063000004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorAndaç, Ahmet Cenk
dc.language.isoenen_US
dc.relation.ispartofJ Chromatogr Scien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleCross referencing 2D-LC determination of intact gliptins in urineen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
andac.pdf
Boyut:
605.18 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

PubMed İndeksli Yayınlar Koleksiyonu
Eczacılık Meslek Bilimleri Bölümü Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu