Epigenetic modulators combination with chemotherapy in breast cancer cells

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dc.authoridEngin Ulukaya / 0000-0003-4875-5472en_US
dc.authorscopusidEngin Ulukaya / 6602927353
dc.authorwosidEngin Ulukaya / K-5792-2018
dc.contributor.authorArı, Ferda
dc.contributor.authorNapieralski, Rudolf
dc.contributor.authorAkgün, Oğuzhan
dc.contributor.authorMagdolen, Viktor
dc.contributor.authorUlukaya, Engin
dc.date.accessioned2021-02-22T10:22:50Z
dc.date.available2021-02-22T10:22:50Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.descriptionPubMed: 33608886en_US
dc.description.abstractDespite the concerning adverse effects on tumour development, epigenetic drugsare very promising in cancer treatment. The aim of this study was to compare the dif-ferential effects of standard chemotherapy regimens (FEC: 5-fluorouracil plus epi-rubicine plus cyclophosphamide) in combination with epigenetic modulators(decitabine, valproic acid): (a) on gene methylation levels of selected tumour bio-markers (LINE-1, uPA, PAI-1, DAPK); (b) their expression status (uPA and PAI-1);(c) differentiation status (5meC and H3K27me3). Furthermore, cell survival as well aschanges concerning the invasion capacity were monitored in cell culture models ofbreast cancer (MCF-7, MDA-MB-231). A significant overall decrease of cell survivalwas observed in the FEC-containing combination therapies for both cell lines. Meth-ylation results showed a general tendency towards increased demethylation of theuPA and PAI-1 gene promoters for the MCF-7 cells, as well as the proapoptoticDAPK gene in the treatment regimens for both cell lines. The uPA and PAI-1 antigenlevels were mainly increased in the supernatant of FEC-only treated MDA-MB-231cells. DAC-only treatment induced an increase of secreted uPA protein in MCF-7 cellculture, while most of the VPA-containing regimens also induced uPA and PAI-1expression in MCF-7 cell fractions. Epigenetically active substances can also induce are-differentiation in tumour cells, as shown by 5meC, H3K27me3 applying ICC.Significance of the study: Epigenetic modulators especially in the highlyundifferentiated and highly malignant MDA-MB-231 tumour cells significantlyreduced tumour malignancy thus; further clinical studies applying specific combina-tion therapies with epigenetic modulators may be warranted.en_US
dc.identifier.citationAri, F., Napieralski, R., Akgun, O., Magdolen, V., & Ulukaya, E. (2021). Epigenetic modulators combination with chemotherapy in breast cancer cells. Cell biochemistry and function, 10.1002/cbf.3626. Advance online publication. https://doi.org/10.1002/cbf.3626en_US
dc.identifier.doi10.1002/cbf.3626en_US
dc.identifier.pmid33608886en_US
dc.identifier.scopus2-s2.0-85101093154en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.1002/cbf.3626
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1486
dc.identifier.wosWOS:000619770600001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorUlukaya, Engin
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofCell Biochem Funct.en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBreast Canceren_US
dc.subjectDNA-Methylationen_US
dc.subjectEpigenetic Modulatorsen_US
dc.subjectHDAC-İnhibitoen_US
dc.titleEpigenetic modulators combination with chemotherapy in breast cancer cellsen_US
dc.typeArticleen_US

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