The improved killing of both androgen-dependent and independent prostate cancer cells by etoposide loaded SPIONs coupled with NIR irradiation

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dc.authoridMerve Erkısa Genel / 0000-0002-3127-742Xen_US
dc.authoridEngin Ulukaya / 0000-0003-4875-5472en_US
dc.authorscopusidEngin Ulukaya / 6602927353
dc.authorscopusidMerve Erkısa Genel / 57126208900
dc.authorwosidMerve Erkısa Genel / AAM-1001-2020en_US
dc.authorwosidEngin Ulukaya / K-5792-2018en_US
dc.contributor.authorOnbaşlı, Kübra
dc.contributor.authorDemirci, Gözde
dc.contributor.authorMuti, Abdullah
dc.contributor.authorSennaroğlu, Alphan
dc.contributor.authorYağcı Acar, Havva
dc.contributor.authorErkısa Genel, Merve
dc.contributor.authorUlukaya, Engin
dc.date.accessioned2022-06-24T07:34:32Z
dc.date.available2022-06-24T07:34:32Z
dc.date.issued2022en_US
dc.departmentISU, Rektörlük, Uygulama ve Araştırma Merkezleri, Moleküler Kanser Uygulama ve Araştırma Merkezien_US
dc.description.abstractEtoposide (Eto) is a toxic drug that shows promise in treating prostate cancer (PCa) but confers significant side effects, and has poor solubility and bioavailability. Nanoparticles are quite successful in overcoming such problems. Multifunctional nanoparticles that provide an opportunity to perform combination therapy have attracted great interest in recent years. Superparamagnetic iron oxide nanoparticles (SPIONs) are popular in various biomedical applications, including magnetic resonance imaging, drug delivery, magnetic hyperthermia and recently in photothermal therapy, combining imaging with therapy. Here, for the enhanced killing of PCa cells that are either androgen-dependent or not, the combination of SPION based Eto delivery and mild hyperthermia triggered by laser irradiation is proposed for the first time in the literature. For the encapsulation of Eto, highly stable, small, polyacrylic acid coated SPIONs were conjugated with bovine serum albumin (BSA) (Eto-BSA@PAA@SPION). Eto-BSA@PAA@SPION with 9% drug content produced better chemotherapeutic outcomes than free Eto on both androgen-dependent/castration sensitive LNCaP and androgen-independent/castration-resistant PC3 and DU145 PCa cells by enhancing drug internalization. Single and short irradiation of Eto-BSA@PAA@SPION treated cells at 808 nm improved the drug release and sensitized cells for Eto, hence, increasing the toxicity dramatically in all studied PCa cell lines. Caspase-mediated apoptosis, DNA damage, and ROS generation were detected in the treated cells, increasing with the Eto dose and laser treatment. The IC50 for Eto is reduced to 0.08 ?g mL-1, 0.13 ?g mL-1 and 2.8 ?g mL-1 with laser/Eto-BSA@PAA@SPION for LNCaP, DU145 and PC3 cells, respectively. These are the lowest IC50 values seen in the literature for Eto on these cell lines so far, suggesting that the demonstrated nanoparticles and treatment approaches have great potential to treat various PCa cells at low doses of the drug under mild laser treatment conditions.en_US
dc.identifier.citationOnbasli K, Erkısa M, Demirci G, Muti A, Ulukaya E, Sennaroglu A, Yagci Acar H. The improved killing of both androgen-dependent and independent prostate cancer cells by etoposide loaded SPIONs coupled with NIR irradiation. Biomater Sci. 2022 Jun 15. doi: 10.1039/d2bm00107a. Epub ahead of print. PMID: 35703472.en_US
dc.identifier.doi10.1039/d2bm00107aen_US
dc.identifier.issn2047-4830en_US
dc.identifier.pmid35703472en_US
dc.identifier.scopus2-s2.0-85132398829en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.uriuri:http://doi.org/10.1039/d2bm00107a
dc.identifier.urihttps://hdl.handle.net/20.500.12713/2937
dc.identifier.wosWOS:000811031300001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorErkısa Genel, Merve
dc.institutionauthorUlukaya, Engin
dc.language.isoenen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.ispartofBiomaterials Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleThe improved killing of both androgen-dependent and independent prostate cancer cells by etoposide loaded SPIONs coupled with NIR irradiationen_US
dc.typeArticleen_US

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