Effects of Lucilia sericata on wound healing in streptozotocin-induced diabetic rats and analysis of its secretome at the proteome level

Basit Öğe Kaydı
dc.authoridFatma Kübra Tombultürk / 0000-0002-4358-2309en_US
dc.authorscopusidFatma Kübra Tombultürk / 56748052500
dc.authorwosidFatma Kübra Tombultürk / L-3969-2018
dc.contributor.authorTombultürk, Fatma Kübra
dc.contributor.authorKasap, Murat
dc.contributor.authorTunçdemir, Matem
dc.contributor.authorPolat, Erdal
dc.contributor.authorSirekbasan, Serhat
dc.contributor.authorKanlı, Ali İsmet
dc.contributor.authorKanıgür-Sultuybek, Gönül
dc.date.accessioned2020-08-30T20:07:36Z
dc.date.available2020-08-30T20:07:36Z
dc.date.issued2018
dc.departmentİstinye Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Laboratuvar Teknikleri Bölümüen_US
dc.description.abstractThe use of Lucilia sericata larvae on the healing of wounds in diabetics has been reported. However, the role of the excretion/secretion (ES) products of the larvae in treatment of diabetic wounds remains unknown. This study investigated whether application of the ES products of L. sericata on the wound surface could improve the impaired wound healing in streptozotocin-induced diabetic rats. Additional analysis was performed to understand proteome content of L. sericata secretome to understand ES contribution at the molecular level. For this purpose, full-thickness skin wounds were created on the backs of diabetic and control rats. A study was conducted to assess the levels of the ES-induced collagen I/III expression and to assay nuclear factor B (NF-B) (p65) activity in wound biopsies and ES-treated wounds of diabetic rat skin in comparison to the controls. The expression levels of collagen I/III and NF-B (p65) activity were determined at days 3, 7, and 14 after wounding using immunohistological analyses and enzyme-linked immunosorbent assay technique. The results indicated that treatment with the ES extract increased collagen I expressions of the wound control and diabetic tissue. But the increase in collagen I expression in the controls was higher than the one in the diabetics. NF-B (p65) activity was also increased in diabetic wounds compared to the controls, whereas it was decreased in third and seventh days upon ES treatment. The results indicated that ES products of L. sericata may enhance the process of wound healing by influencing phases such as inflammation, NF-B (p65) activity, collagen synthesis, and wound contraction. These findings may provide new insights into understanding of therapeutic potential of ES in wound healing in diabetics.en_US
dc.description.sponsorshipResearch Fund of The University of IstanbulIstanbul University [35048]en_US
dc.description.sponsorshipThis study was supported by the Research Fund of The University of Istanbul (Project Number 35048). We appreciate this support.en_US
dc.identifier.citationTombulturk, F. K., Kasap, M. U. R. A. T., Tuncdemir, M., Polat, E., Sirekbasan, S., Kanli, A. Y. L. İ. N., & Kanigur-Sultuybek, G. (2018). Effects of Lucilia sericata on wound healing in streptozotocin-induced diabetic rats and analysis of its secretome at the proteome level. Human & experimental toxicology, 37(5), 508-520.en_US
dc.identifier.doi10.1177/0960327117714041en_US
dc.identifier.endpage520en_US
dc.identifier.issn0960-3271en_US
dc.identifier.issn1477-0903en_US
dc.identifier.issue5en_US
dc.identifier.pmid28621210en_US
dc.identifier.scopus2-s2.0-85044974863en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage508en_US
dc.identifier.urihttps://doi.org/10.1177/0960327117714041
dc.identifier.urihttps://hdl.handle.net/20.500.12713/796
dc.identifier.volume37en_US
dc.identifier.wosWOS:000429812900007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorTombultürk, Fatma Kübraen_US
dc.language.isoenen_US
dc.publisherSage Publications Ltden_US
dc.relation.ispartofHuman & Experimental Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectNuclear Factor -Ben_US
dc.subjectCollagenen_US
dc.subjectStz Diabetesen_US
dc.subjectMaggot Debridement Therapyen_US
dc.subjectLucilia Sericataen_US
dc.titleEffects of Lucilia sericata on wound healing in streptozotocin-induced diabetic rats and analysis of its secretome at the proteome levelen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
115.pdf
Boyut:
824.41 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Tıbbi Laboratuvar Teknikleri Programı Makale Koleksiyonu