Synthesis, Characterization and Apoptosis Capabilities of a Novel Naphthoquinone-Derived Compound in Triple-Negative Breast Cancer
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Breast cancer is the most frequently diagnosed cancer in women and the second leading cause of cancer-related deaths. Because triple-negative breast cancer (TNBC) is highly resistant to clinically employed drugs, developing new drugs and treatment approaches is imperative. Naphthoquinone compounds potentially induce cancer cell death, and their clinical derivatives have been widely used as medicines. This study presents the synthesis and structure of a newly substituted naphthoquinone compound, as determined by spectroscopic techniques, then assesses the compound's ability to induce cell death on the MDA-MB-231 cell line using SRB viability testing, flow cytometry, and Western Blotting. The study also examines the compound's inhibition of cell migration through a scratch assay. The study discovered that the compound demonstrated an antigrowth/cytotoxic impact at concentrations of 3.12 mu M and beyond. The cytotoxicity may have been caused by elevated reactive oxygen species, which resulted in DNA damage. The mode of cell death was apoptotic, confirmed by the translocation of phosphatidylserine, the activation of caspases, and the disruption of the mitochondrial membrane potential. The compound seems promising as a new anti-malignant agent to be used for the treatment of breast cancer and deserves further attention for proof-of-concept in animal studies. Breast cancer, the most prevalent female cancer and second-leading cancer cause of death, includes the aggressive TNBC variant. Naphthoquinone compounds show promise in TNBC treatment. Recent research reveals a novel naphthoquinone's potential in inducing apoptosis at concentrations >= 3.12 mu M, possibly via ROS elevation, DNA damage, and apoptotic cell death. Its ability to hinder cell migration suggests anti-metastatic properties. Yet, thorough research, including animal model validation and human safety and efficacy assessments, is crucial before deeming it a viable TNBC treatment.image
Açıklama
Anahtar Kelimeler
Naphthoquinone, Cytotoxicity, Genotoxicity, Anti-Cancer Drug, Chemotherapy, Mammary Tumor
Kaynak
Chemistryselect
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
8
Sayı
43
