A rat study on the PTEN expression in ovarian tissue in PCOS and folliculogenesis

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dc.authorwosidKalem, Muberra Namlı/O-9531-2017
dc.contributor.authorNamli Kalem, Muberra
dc.contributor.authorAnadol, Elvan
dc.contributor.authorKalem, Ziya
dc.contributor.authorSezginer, Perihan Yalcinkaya
dc.contributor.authorElmas, Cigdem
dc.contributor.authorYilmaz, Canan
dc.contributor.authorBakirarar, Batuhan
dc.date.accessioned2024-05-19T14:50:36Z
dc.date.available2024-05-19T14:50:36Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractThe objective of this investigation was to examine alterations in PTEN expression within ovarian tissue in a rat model of polycystic ovary syndrome (PCOS). The analysis also encompassed the examination of PTEN alterations in the ovarian tissue throughout the process of folliculogenesis in rats with normal ovulatory cycles. The study involved 12 adult female Sprague-Dawley rats randomly assigned to the letrozole-induced polycystic ovary syndrome (PCOS) group as part of an animal-based research endeavour. The sections derived from the ovaries were subjected to immunohistochemical staining for PTEN. The evaluation of PTEN staining levels in ovarian tissues was conducted using electron microscopy. Follicle counts, as well as hormonal and biochemical analyses (serum luteinising hormone (LH), follicle-stimulating hormone (FSH), anti-Mullerian hormone (AMH), testosterone, oestradiol levels and serum glucose, triglyceride, HDL and LDL-cholesterol levels), were conducted to provide evidence of the manifestation of polycystic ovary syndrome (PCOS) in rats. The number of primordial and Graafian follicles in the PCOS group decreased significantly, and the number of primary, secondary and antral follicles increased significantly. PTEN expression was found to be significantly higher in the PCOS group than in the control group in the primordial follicle oocyte cytoplasm, primordial follicle granulosa cells, primary follicle oocyte cytoplasm, primary follicle granulosa cells, antral follicle oocyte cytoplasm, antral follicle granulosa cells, and corpus luteum (p = 0.007, p = 0.001, p = 0.001, p = 0.001, p = 0.001, p = 0.002, and p = 0.018, respectively). In the non-PCOS group, a time-dependent comparison of the amount of oocyte cytoplasm and PTEN staining in granulosa cells of the oocytes at different stages of development was performed. While the follicles were developing from the primordial follicle to the primary and antral follicle, the amount of PTEN staining in the oocyte cytoplasm decreased, whereas the PTEN activity in the granulosa cells increased as the oocyte developed (p = 0.001 and p = 0.001, respectively). The current investigation demonstrated changes in PTEN expression in ovarian tissue throughout the course of normal folliculogenesis, as well as in instances of disrupted folliculogenesis, with a focus on rats with PCOS.en_US
dc.identifier.doi10.1038/s41598-023-47809-y
dc.identifier.issn2045-2322
dc.identifier.issue1en_US
dc.identifier.pmid38008769en_US
dc.identifier.scopus2-s2.0-85177731850en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org10.1038/s41598-023-47809-y
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5756
dc.identifier.volume13en_US
dc.identifier.wosWOS:001124265200003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherNature Portfolioen_US
dc.relation.ispartofScientific Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectGranulosa-Cellsen_US
dc.subjectPathwayen_US
dc.subjectGrowthen_US
dc.subjectDifferentiationen_US
dc.subjectProliferationen_US
dc.subjectFshen_US
dc.titleA rat study on the PTEN expression in ovarian tissue in PCOS and folliculogenesisen_US
dc.typeArticleen_US

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