Preparation of glutathione loaded nanoemulsions and testing of hepatoprotective activity on THLE-2 cells
Yükleniyor...
Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
To improve bioavailability and stability of hydrophobic and hydrophilic compounds, nanoemulsions are good alternatives as delivery systems because of their nontoxic and nonirritant nature. Glutathione (GSH) suffers from low stability in water, where its encapsulation in nanoemulsions is a powerful strategy to its stability in aqueous systems. The aim of this study was to obtain nanoemulsions from the hydrophobic/hydrophilic contents of N. sativa seed oil so as to improve GSH stability along with bioavailability of N. sativa seed oil. Then, the prepared nanoemulsions were tested for in vitro hepatoprotective activity against ethanol toxicity. To the best of our knowledge, there is no study on the test of nanoemulsions by the combination of Nigella sativa seed oils and GSH in hepatoprotective activity. Here, nanoemulsions with different contents were prepared using Nigella sativa seed oils. Content analyses and characterisation studies of prepared nanoemulsions were carried out. In order to investigate the protective effects against to ethanol exposure, THLE-2 cells were pretreated with nanoemulsions for 2 h with the maximum benign dose (0.5 mg/mL of nanoemulsions). Ethanol (400 mM) was introduced to pretreated cells and nontreated cells for 48- or 72-h periods, followed by cell viability assay was carried out. Fluorescence microscopy tests revealed the introduction of the nanoemulsions into THLE-2 cells. The findings show that nanoformulations have promising in vitro hepatoprotective effects on the THLE-2 cell line against ethanol exposure.
Açıklama
Anahtar Kelimeler
Ethanol, Nigella Sativa, Glutathione, Nanoemulsion, Hepatoprotective Activity, Nigella-Sativa, Antioxidant Properties, Colorimetric Assay, Gold Nanoparticles, Ethanol, Seed, Oil, Metabolism
Kaynak
TURKISH JOURNAL OF CHEMISTRY
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
45
Sayı
2
Künye
YEŞİLTEPE, O., ÇELİK, E. G., GEYİK, C., GÜMÜŞ, Z. P., DEMİRKOL, D. O., COŞKUNOL, H., & TİMUR, S. (2021). Preparation of glutathione loaded nanoemulsions and testing of hepatoprotective activity on THLE-2 cells. Turkish Journal of Chemistry, 45(2), 436-451.
