Bioassay-guided isolation of cytotoxic compounds from chrysophthalmum montanum (DC.) boiss

dc.authoridEngin Ulukaya / 0000-0003-4875-5472en_US
dc.authorscopusidEngin Ulukaya / 6602927353
dc.authorwosidEngin Ulukaya / K-5792-2018
dc.contributor.authorAyaz, Fatma
dc.contributor.authorKüçükkboyacı, Nurgün
dc.contributor.authorGören, Nezhun
dc.contributor.authorÇalış, İhsan
dc.contributor.authorAydınlık, Şeyma
dc.contributor.authorUlukaya, Engin
dc.contributor.authorDuman, Hayri
dc.contributor.authorChoudhary, Muhammad Iqbal
dc.date.accessioned2020-08-30T20:06:55Z
dc.date.available2020-08-30T20:06:55Z
dc.date.issued2019
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractBioassay-guided isolation of the 80% methanol extract of the aerial parts of Chrysophthalmum montanum (DC.) Boiss. (Asteraceae) led to the isolation of four known guaianolide-type sesquiterpene lactones, 6 alpha-acetoxy-4 alpha-hydroxy-113H-guaia-9.11(13)-dien-12.8a-olide (1), 6 alpha-acetoxy-4 alpha-hydroxy-9 beta.10 beta-epoxy-1 beta H-guaia-11(13)-en12.8 alpha-olide (2), 4 alpha,6 alpha-dihydroxy-1 beta H,5 alpha,7 alpha H-guaia-9(10),11(13)-dien-12,8 alpha-olide (3), and (4 alpha,5 alpha,8 beta,10 beta)-4,10-dihydroxy-1,11(13)-guaidien-12,8-olide (4), along a steroidal glycoside mixture (5a and 5b). The structures of the compounds were identified on the basis of spectroscopic data. Among them, 2, 4 and a steroidal glycoside mixture were obtained from C. montanum for the first time. All isolates were also first time assayed for in vitro cytotoxicities against four human cancer cell lines, i.e. breast (MCF-7, MDA-MB 231), colon (FIT-29), and lung (PC3). Among the isolates, 1-3 showed significant inhibitory effect on the proliferation of cancer cells with viability ranging from 6.86 to 26.51%, while steroidal glycoside mixture showed no cytotoxicity, except against HT-29 (viability 61.99%). Compound 4 exhibited strong and selective cell growth inhibition against HT-29 with viability 20.99% and was identified as a promising compound with high selectivity between cancer cells and normal human lung cells (SEAS-2B), especially against HT-29 (IC50 = 12.2 pg/mL) compared to that of cisplatin. These results suggested that 4 is worthy of further study to determine its cytotoxicity mechanisms.en_US
dc.description.sponsorshipGazi University Research FoundationGazi University [02/2017-03]; Scientific and Technological Research Council of Turkey (TUBITAK) from TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [2211/A, 2214/A]; International Center for Chemical and Bological Sciences (International Center for Chemical and Bological Sciences (ICCBS)-Hussain Ebrahim Jamal Research Institute of Chemistry from Pakistanen_US
dc.description.sponsorshipThis study was supported by Gazi University Research Foundation [grant number 02/2017-03],The Scientific and Technological Research Council of Turkey (TUBITAK) 2211/A and 2214/A, from Turkey, as well as International Center for Chemical and Bological Sciences (International Center for Chemical and Bological Sciences (ICCBS)-Hussain Ebrahim Jamal Research Institute of Chemistry from Pakistan.en_US
dc.identifier.citationAyaz, F., Küçükboyacı, N., Gören, N., Çalış, İ., Aydınlık, Ş., Ulukaya, E., ... & Choudhary, M. I. (2019). Bioassay-guided isolation of cytotoxic compounds from Chrysophthalmum montanum (DC.) Boiss. Food and Chemical Toxicology, 125, 10-20.en_US
dc.identifier.doi10.1016/j.fct.2018.12.029en_US
dc.identifier.endpage20en_US
dc.identifier.issn0278-6915en_US
dc.identifier.issn1873-6351en_US
dc.identifier.pmid30580030en_US
dc.identifier.scopus2-s2.0-85059058009en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage10en_US
dc.identifier.urihttps://doi.org/10.1016/j.fct.2018.12.029
dc.identifier.urihttps://hdl.handle.net/20.500.12713/652
dc.identifier.volume125en_US
dc.identifier.wosWOS:000463305000002en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorUlukaya, Enginen_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofFood and Chemical Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChrysophthalmum Montanumen_US
dc.subjectAsteraceaeen_US
dc.subjectSesquiterpene Lactonesen_US
dc.subjectSteroidal Glycosidesen_US
dc.subjectCytotoxicityen_US
dc.subjectHt-29 Colon Cancer Cell Lineen_US
dc.titleBioassay-guided isolation of cytotoxic compounds from chrysophthalmum montanum (DC.) boissen_US
dc.typeArticleen_US

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