Bioassay-guided isolation of cytotoxic compounds from chrysophthalmum montanum (DC.) boiss
dc.authorid | Engin Ulukaya / 0000-0003-4875-5472 | en_US |
dc.authorscopusid | Engin Ulukaya / 6602927353 | |
dc.authorwosid | Engin Ulukaya / K-5792-2018 | |
dc.contributor.author | Ayaz, Fatma | |
dc.contributor.author | Küçükkboyacı, Nurgün | |
dc.contributor.author | Gören, Nezhun | |
dc.contributor.author | Çalış, İhsan | |
dc.contributor.author | Aydınlık, Şeyma | |
dc.contributor.author | Ulukaya, Engin | |
dc.contributor.author | Duman, Hayri | |
dc.contributor.author | Choudhary, Muhammad Iqbal | |
dc.date.accessioned | 2020-08-30T20:06:55Z | |
dc.date.available | 2020-08-30T20:06:55Z | |
dc.date.issued | 2019 | |
dc.department | İstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü | en_US |
dc.description.abstract | Bioassay-guided isolation of the 80% methanol extract of the aerial parts of Chrysophthalmum montanum (DC.) Boiss. (Asteraceae) led to the isolation of four known guaianolide-type sesquiterpene lactones, 6 alpha-acetoxy-4 alpha-hydroxy-113H-guaia-9.11(13)-dien-12.8a-olide (1), 6 alpha-acetoxy-4 alpha-hydroxy-9 beta.10 beta-epoxy-1 beta H-guaia-11(13)-en12.8 alpha-olide (2), 4 alpha,6 alpha-dihydroxy-1 beta H,5 alpha,7 alpha H-guaia-9(10),11(13)-dien-12,8 alpha-olide (3), and (4 alpha,5 alpha,8 beta,10 beta)-4,10-dihydroxy-1,11(13)-guaidien-12,8-olide (4), along a steroidal glycoside mixture (5a and 5b). The structures of the compounds were identified on the basis of spectroscopic data. Among them, 2, 4 and a steroidal glycoside mixture were obtained from C. montanum for the first time. All isolates were also first time assayed for in vitro cytotoxicities against four human cancer cell lines, i.e. breast (MCF-7, MDA-MB 231), colon (FIT-29), and lung (PC3). Among the isolates, 1-3 showed significant inhibitory effect on the proliferation of cancer cells with viability ranging from 6.86 to 26.51%, while steroidal glycoside mixture showed no cytotoxicity, except against HT-29 (viability 61.99%). Compound 4 exhibited strong and selective cell growth inhibition against HT-29 with viability 20.99% and was identified as a promising compound with high selectivity between cancer cells and normal human lung cells (SEAS-2B), especially against HT-29 (IC50 = 12.2 pg/mL) compared to that of cisplatin. These results suggested that 4 is worthy of further study to determine its cytotoxicity mechanisms. | en_US |
dc.description.sponsorship | Gazi University Research FoundationGazi University [02/2017-03]; Scientific and Technological Research Council of Turkey (TUBITAK) from TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [2211/A, 2214/A]; International Center for Chemical and Bological Sciences (International Center for Chemical and Bological Sciences (ICCBS)-Hussain Ebrahim Jamal Research Institute of Chemistry from Pakistan | en_US |
dc.description.sponsorship | This study was supported by Gazi University Research Foundation [grant number 02/2017-03],The Scientific and Technological Research Council of Turkey (TUBITAK) 2211/A and 2214/A, from Turkey, as well as International Center for Chemical and Bological Sciences (International Center for Chemical and Bological Sciences (ICCBS)-Hussain Ebrahim Jamal Research Institute of Chemistry from Pakistan. | en_US |
dc.identifier.citation | Ayaz, F., Küçükboyacı, N., Gören, N., Çalış, İ., Aydınlık, Ş., Ulukaya, E., ... & Choudhary, M. I. (2019). Bioassay-guided isolation of cytotoxic compounds from Chrysophthalmum montanum (DC.) Boiss. Food and Chemical Toxicology, 125, 10-20. | en_US |
dc.identifier.doi | 10.1016/j.fct.2018.12.029 | en_US |
dc.identifier.endpage | 20 | en_US |
dc.identifier.issn | 0278-6915 | en_US |
dc.identifier.issn | 1873-6351 | en_US |
dc.identifier.pmid | 30580030 | en_US |
dc.identifier.scopus | 2-s2.0-85059058009 | en_US |
dc.identifier.scopusquality | Q1 | en_US |
dc.identifier.startpage | 10 | en_US |
dc.identifier.uri | https://doi.org/10.1016/j.fct.2018.12.029 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12713/652 | |
dc.identifier.volume | 125 | en_US |
dc.identifier.wos | WOS:000463305000002 | en_US |
dc.identifier.wosquality | Q1 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.indekslendigikaynak | PubMed | en_US |
dc.institutionauthor | Ulukaya, Engin | en_US |
dc.language.iso | en | en_US |
dc.publisher | Pergamon-Elsevier Science Ltd | en_US |
dc.relation.ispartof | Food and Chemical Toxicology | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Chrysophthalmum Montanum | en_US |
dc.subject | Asteraceae | en_US |
dc.subject | Sesquiterpene Lactones | en_US |
dc.subject | Steroidal Glycosides | en_US |
dc.subject | Cytotoxicity | en_US |
dc.subject | Ht-29 Colon Cancer Cell Line | en_US |
dc.title | Bioassay-guided isolation of cytotoxic compounds from chrysophthalmum montanum (DC.) boiss | en_US |
dc.type | Article | en_US |
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